PUBLICATION
Fin ray branching is defined by TRAP+ osteolytic tubules in zebrafish
- Authors
- Cardeira-da-Silva, J., Bensimon-Brito, A., Tarasco, M., Brandão, A.S., Rosa, J.T., Borbinha, J., Almeida, P.J., Jacinto, A., Cancela, M.L., Gavaia, P.J., Stainier, D.Y.R., Laizé, V.
- ID
- ZDB-PUB-221124-5
- Date
- 2022
- Source
- Proceedings of the National Academy of Sciences of the United States of America 119: e2209231119e2209231119 (Journal)
- Registered Authors
- Bensimon-Brito, Anabela, Stainier, Didier
- Keywords
- bifurcation, bone patterning, bone resorption, fin regeneration, osteoclasts
- MeSH Terms
-
- Animals
- Bone and Bones/metabolism
- Osteoblasts/metabolism
- Signal Transduction
- Zebrafish*/metabolism
- Zebrafish Proteins*/metabolism
- PubMed
- 36417434 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Cardeira-da-Silva, J., Bensimon-Brito, A., Tarasco, M., Brandão, A.S., Rosa, J.T., Borbinha, J., Almeida, P.J., Jacinto, A., Cancela, M.L., Gavaia, P.J., Stainier, D.Y.R., Laizé, V. (2022) Fin ray branching is defined by TRAP+ osteolytic tubules in zebrafish. Proceedings of the National Academy of Sciences of the United States of America. 119:e2209231119e2209231119.
Abstract
The shaping of bone structures relies on various cell types and signaling pathways. Here, we use the zebrafish bifurcating fin rays during regeneration to investigate bone patterning. We found that the regenerating fin rays form via two mineralization fronts that undergo an osteoblast-dependent fusion/stitching until the branchpoint, and that bifurcation is not simply the splitting of one unit into two. We identified tartrate-resistant acid phosphatase-positive osteolytic tubular structures at the branchpoints, hereafter named osteolytic tubules (OLTs). Chemical inhibition of their bone-resorbing activity strongly impairs ray bifurcation, indicating that OLTs counteract the stitching process. Furthermore, by testing different osteoactive compounds, we show that the position of the branchpoint depends on the balance between bone mineralization and resorption activities. Overall, these findings provide a unique perspective on fin ray formation and bifurcation, and reveal a key role for OLTs in defining the proximo-distal position of the branchpoint.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping