PUBLICATION
Adverse effects of polystyrene nanoplastic and its binary mixtures with nonylphenol on zebrafish nervous system: From oxidative stress to impaired neurotransmitter system
- Authors
- Aliakbarzadeh, F., Rafiee, M., Khodagholi, F., Khorramizadeh, M.R., Manouchehri, H., Eslami, A., Sayehmiri, F., Mohseni-Bandpei, A.
- ID
- ZDB-PUB-221108-1
- Date
- 2022
- Source
- Environmental pollution (Barking, Essex : 1987) 317: 120587 (Journal)
- Registered Authors
- Khorramizadeh, M.Reza
- Keywords
- Nanoplastics, Neurotransmitter system, Nonylphenol (4-NP), Oxidative stress, Zebrafish (Danio rerio)
- MeSH Terms
-
- Acetylcholinesterase/metabolism
- Animals
- Antioxidants/metabolism
- Microplastics/metabolism
- Nanoparticles*/toxicity
- Nervous System/metabolism
- Oxidative Stress
- Plastics/metabolism
- Polystyrenes/metabolism
- Water Pollutants, Chemical*/toxicity
- Zebrafish/metabolism
- PubMed
- 36336178 Full text @ Environ. Pollut.
Citation
Aliakbarzadeh, F., Rafiee, M., Khodagholi, F., Khorramizadeh, M.R., Manouchehri, H., Eslami, A., Sayehmiri, F., Mohseni-Bandpei, A. (2022) Adverse effects of polystyrene nanoplastic and its binary mixtures with nonylphenol on zebrafish nervous system: From oxidative stress to impaired neurotransmitter system. Environmental pollution (Barking, Essex : 1987). 317:120587.
Abstract
Micro(nano)plastics generally co-exist with other chemicals in the environment, resulting in inevitable interaction and combined toxic effects on biota. Nevertheless, little is known regarding the interaction of nanoplastics (NPs) with other co-occurring insults. Hereby, we investigated single and combined effects of chronic exposure (45 days) to polystyrene nanoplastic particulates (PS-NPs) and nonylphenol (4-NP) on zebrafish nervous system. Multiple biomarkers concerning with oxidative-stress [catalase (CAT) activity and reduced glutathione (GSH) level], cholinergic system [Acetylcholinesterase (AchE) activity], glutamatergic system [glutamine synthetase (GS) and glutamate dehydrogenase (GDH) activities], energy metabolism [a-ketoglutarate dehydrogenase (a-KGDH) activity], and histological alterations were assessed. Both single and binary exposure to PS-NPs and 4-NP induced oxidative stress through reducing CAT activity and GSH level, in which a more sever effect was noticed in combined exposure. The AchE activity was significantly inhibited only in single treatment groups demonstrating antagonistic interaction between PS-NPs and 4-NP. Effects on GS activity was also alleviated in binary exposure as compared with single exposure to each contaminant. In addition, an increase in GDH activity was noticed in PS-NPs at 10 and 100 μg/L, and simultaneous presence of PS-NPs and 4-NP with a greater response were observed in combined treatments. PS-NPs and 4-NP either in separate or binary mixtures disrupted energy metabolism by deficiency of α-KGDH activity; however, co-exposure to PS-NPs and 4-NP induced more intense adverse impacts on this parameter. Furthermore, histological analysis revealed that 4-NP and PS-NPs, alone or in combination, reduced neural cells. These findings provide new insight into the neurotoxic effects of binary exposure to PS-NPs and 4-NP at environmentally relevant concentrations. Overall, our findings raise concerns about the presence and toxicity of nano-scale plastic particulates and highlight the importance of investigating the interaction of Micro(nano)plastics with other environmental irritants.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping