PUBLICATION

Rhabdomyosarcoma xenotransplants in zebrafish embryos

Authors
Siebert, J., Schneider, M., Reuter-Schmitt, D., Würtemberger, J., Neubüser, A., Driever, W., Hettmer, S., Kapp, F.G.
ID
ZDB-PUB-221102-3
Date
2022
Source
Pediatric blood & cancer   70(1): e30053 (Journal)
Registered Authors
Driever, Wolfgang, Kapp, Friedrich
Keywords
RMS, rhabdomyosarcoma, xenograft, zebrafish embryo
MeSH Terms
  • Animals
  • Child
  • Heterografts
  • Humans
  • Mammals
  • Rhabdomyosarcoma*/drug therapy
  • Rhabdomyosarcoma*/pathology
  • Rhabdomyosarcoma, Embryonal*/drug therapy
  • Xenograft Model Antitumor Assays
  • Zebrafish
PubMed
36317680 Full text @ Pediatr Blood Cancer
Abstract
Rhabdomyosarcomas (RMS) are the most common pediatric soft tissue sarcomas. High-risk and metastatic disease continues to be associated with very poor prognosis. RMS model systems that faithfully recapitulate the human disease and provide rapid, cost-efficient estimates of antitumor efficacy of candidate drugs are needed to facilitate drug development and personalized medicine approaches. Here, we present a new zebrafish-based xenotransplant model allowing for rapid and easily accessible drug screening using low numbers of viable tumor cells and relatively small amounts of water-soluble chemicals. Under optimized temperature conditions, embryonal RMS xenografts were established in zebrafish embryos at 3 h postfertilization (hpf). In proof-of-principle experiments, chemotherapy drugs with established clinical anti-RMS efficacy (vincristine, dactinomycin) and the mitogen-activated protein kinase kinase inhibitor trametinib were shown to significantly reduce the cross-sectional area of the tumors by 120 hpf. RMS xenograft models in zebrafish embryos henceforth could serve as a valuable addition to cell culture and mammalian models of RMS and represent a rapid and cost-effective solution for preclinical candidate drug testing.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping