PUBLICATION
Clionasterol-Rich Fraction of Caulerpa racemosa against Particulate Matter-Induced Skin Damage via Inhibition of Oxidative Stress and Apoptosis-Related Signaling Pathway
- Authors
- Liyanage, N.M., Nagahawatta, D.P., Jayawardena, T.U., Jayawardhana, H.H.A.C.K., Lee, H.G., Kim, Y.S., Jeon, Y.J.
- ID
- ZDB-PUB-221028-14
- Date
- 2022
- Source
- Antioxidants (Basel, Switzerland) 11(10): (Journal)
- Registered Authors
- Jeon, You-Jin
- Keywords
- Caulerpa racemose, apoptosis, clionasterol, mitochondria, oxidative stress, particulate matter, zebrafish model
- MeSH Terms
- none
- PubMed
- 36290664 Full text @ Antioxidants (Basel)
Citation
Liyanage, N.M., Nagahawatta, D.P., Jayawardena, T.U., Jayawardhana, H.H.A.C.K., Lee, H.G., Kim, Y.S., Jeon, Y.J. (2022) Clionasterol-Rich Fraction of Caulerpa racemosa against Particulate Matter-Induced Skin Damage via Inhibition of Oxidative Stress and Apoptosis-Related Signaling Pathway. Antioxidants (Basel, Switzerland). 11(10):.
Abstract
The increasing airborne particulate matter (PM) consisting of environmental contaminants such as dust, aerosols, and fibers has become a global concern by causing oxidative stress that leads to apoptosis and skin damage. The current study evaluated the protective effect of Caulerpa racemosa (CR) against PM-induced skin damage using human keratinocytes and a zebrafish model. The clionasterol-rich hexane fraction (CRHF2) of CR exhibited superior protective activity through downregulating intracellular reactive oxygen species levels and mitochondrial ROS levels, as well as the PM-induced increase in apoptotic body formation and upregulation of apoptotic signaling pathway proteins, along with sub-G1 cell accumulation dose-dependently. Furthermore, in vivo results showed that CRHF2 potentially downregulates PM-induced cell death, ROS, and NO production in the zebrafish model. Hence, the results evidenced that the protective effect of CRHF2 is caused by inhibiting oxidative stress and mitochondrial-mediated apoptosis in cells. Therefore, C. racemosa has the potential to be used in the development of pharmaceuticals to attenuate PM-induced skin diseases.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping