PUBLICATION
Arginine-Containing Peptides Derived from Walnut Protein Against Cognitive and Memory Impairment in Scopolamine-Induced Zebrafish: Design, Release, and Neuroprotection
- Authors
- Wang, S., Su, G., Fan, J., Xiao, Z., Zheng, L., Zhao, M., Wu, J.
- ID
- ZDB-PUB-220914-26
- Date
- 2022
- Source
- Journal of Agricultural and Food Chemistry 70(37): 11579-11590 (Journal)
- Registered Authors
- Keywords
- arginine-containing peptides, cognitive and memory impairment, neuroprotection, release, walnut protein
- MeSH Terms
-
- Animals
- Antioxidants/chemistry
- Arginine
- Cognition
- Hydrolysis
- Juglans*/chemistry
- Memory Disorders/chemically induced
- Neuroprotection
- Neuroprotective Agents*/pharmacology
- Papain
- Pepsin A
- Peptides/chemistry
- Scopolamine/adverse effects
- Thermolysin
- Trypsin
- Zebrafish
- PubMed
- 36098553 Full text @ J. Agric. Food Chem.
Citation
Wang, S., Su, G., Fan, J., Xiao, Z., Zheng, L., Zhao, M., Wu, J. (2022) Arginine-Containing Peptides Derived from Walnut Protein Against Cognitive and Memory Impairment in Scopolamine-Induced Zebrafish: Design, Release, and Neuroprotection. Journal of Agricultural and Food Chemistry. 70(37):11579-11590.
Abstract
The purpose of this study was to investigate the neuroprotective effect of Arg-containing peptides from walnut storage protein sequences in scopolamine-induced zebrafish and further to validate the potential neuroprotection of Arg-containing peptide enriched walnut hydrolysates prepared by in silico hydrolysis and controlled enzymatic release. Results showed that walnut derived Arg-containing peptides with high abundance and great bioactivity predicted by bioinformatics displayed potent neuroprotection in scopolamine-induced zebrafish, and regulation of neurotransmitter level and antioxidant enzyme activity might be the main target for Arg-containing peptides to exert neuroprotection. Notably, Arg-containing peptides (not free arginine) contributed greater neuroprotection, and the positive charge and cell-penetrating properties also affected their neuroprotection. Subsequently, Arg-containing peptides could be released efficiently from walnut protein following hydrolysis by trypsin, pepsin, papain, and thermolysin (bound arginine content: ranging from 110.43 ± 1.58 to 121.82 ± 1.02 mg/g). Among them, trypsin had excellent potential for releasing Arg-containing peptides in silico hydrolysis, and its hydrolysate was confirmed to have neuroprotective capacity, indicating that the combination of in silico hydrolysis and controlled enzymatic release might be an effective approach to obtain Arg-containing neuroprotective peptides.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping