PUBLICATION
Prolonged Hyperglycemia Causes Visual and Cognitive Deficits in Danio rerio
- Authors
- McCarthy, E., Dunn, J., Augustine, K., Connaughton, V.P.
- ID
- ZDB-PUB-220911-17
- Date
- 2022
- Source
- International Journal of Molecular Sciences 23(17): (Journal)
- Registered Authors
- Connaughton, Victoria P.
- Keywords
- T2DM, blood sugar, optomotor response, three-chamber choice, zebrafish
- MeSH Terms
-
- Animals
- Cognition
- Cognitive Dysfunction*/etiology
- Diabetes Mellitus, Type 2*
- Glucose
- Hyperglycemia*/complications
- Mannitol/pharmacology
- Water
- Zebrafish/physiology
- PubMed
- 36077569 Full text @ Int. J. Mol. Sci.
Citation
McCarthy, E., Dunn, J., Augustine, K., Connaughton, V.P. (2022) Prolonged Hyperglycemia Causes Visual and Cognitive Deficits in Danio rerio. International Journal of Molecular Sciences. 23(17).
Abstract
The present study induced prolonged hyperglycemia (a hallmark symptom of Type 2 diabetes [T2DM]) in Danio rerio (zebrafish) for eight or twelve weeks. The goal of this research was to study cognitive decline as well as vision loss in hyperglycemic zebrafish. Fish were submerged in glucose for eight or twelve weeks, after which they were assessed with both a cognitive assay (three-chamber choice) and a visual assay (optomotor response (OMR)). Zebrafish were also studied during recovery from hyperglycemia. Here, fish were removed from the hyperglycemic environment for 4 weeks after either 4 or 8 weeks in glucose, and cognition and vision was again assessed. The 8- and 12-week cognitive results revealed that water-treated fish showed evidence of learning while glucose- and mannitol-treated fish did not within the three-day testing period. OMR results identified an osmotic effect with glucose-treated fish having significantly fewer positive rotations than water-treated fish but comparable rotations to mannitol-treated fish. The 8- and 12-week recovery results showed that 4 weeks was not enough time to fully recovery from the hyperglycemic insult sustained.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping