PUBLICATION

Methylome data derived from maternal-zygotic DNA methyltransferase 3aa-/- zebrafish

Authors
Shirai, M., Takayama, K., Takahashi, H., Hirose, Y., Fujii, M., Awazu, A., Shimoda, N., Kikuchi, Y.
ID
ZDB-PUB-220831-9
Date
2022
Source
Data in brief   44: 108514 (Journal)
Registered Authors
Kikuchi, Yutaka, Shimoda, Nobuyoshi
Keywords
DNA methylation, Dnmt3aa, Whole genome bisulfate sequencing, Zebrafish
Datasets
GEO:GSE178690, GEO:GSE178691, GEO:GSE178688
MeSH Terms
none
PubMed
36034642 Full text @ Data Brief
Abstract
Genomic DNA methylation is an epigenetic marker mediated by DNA methyltransferases (Dnmts); in vertebrates, it comprises of a maintenance DNA methyltransferase, Dnmt1, and two de novo DNA methyltransferases (Dnmt3a and Dnmt3b). In zebrafish, there are two homologs of the mammalian Dnmt3a: Dnmt3aa and Dnmt3ab. A knockout (KO) mutant of zebrafish dnmt3aa was generated using the CRISPR/Cas9 genome-editing system as a new model for DNA methylation research. Since zebrafish dnmt3aa KO mutants were viable and fertile, a maternal-zygotic dnmt3aa deficient mutant (MZdnmt3aa) was generated. We performed whole-genome bisulfite sequencing (WGBS) to reveal the DNA methylation profile using this mutant and identified genomic regions with altered CpG methylation as differentially methylated regions (DMRs) in this mutant compared to those in the wild-type fish. We provided novel raw and processed datasets using the MZdnmt3aa KO mutant, and the raw data of WGBS are available through the Gene Expression Omnibus (GEO), accession number GSE178690.
Genes / Markers
Figures
Figure Gallery (1 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
dnmt3aazf3681/zf3681standard conditionsLong-pec
1 - 1 of 1
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
zf3681
    		Small Deletion
    1 - 1 of 1
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    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    dnmt3aaCRISPR5-dnmt3aaCRISPR
    dnmt3aaCRISPR6-dnmt3aaCRISPR
    dnmt3aaCRISPR7-dnmt3aaCRISPR
    1 - 3 of 3
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    Fish
    Fish
    dnmt3aazf3681/zf3681
    1 - 1 of 1
    Show
    Antibodies
    No data available
    Orthology
    No data available
    Engineered Foreign Genes
    No data available
    Mapping
    No data available
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    Citation
    Shirai, M., Takayama, K., Takahashi, H., Hirose, Y., Fujii, M., Awazu, A., Shimoda, N., Kikuchi, Y. (2022) Methylome data derived from maternal-zygotic DNA methyltransferase 3aa-/- zebrafish. Data in brief. 44:108514.