PUBLICATION
The Expression and Function of lincRNA-154324 and the Adjoining Protein-Coding Gene vmp1 in the Caudal Fin Regeneration of Zebrafish
- Authors
- Li, J., Wen, W., Zhang, S., Zhou, C., Feng, Y., Li, X.
- ID
- ZDB-PUB-220827-18
- Date
- 2022
- Source
- International Journal of Molecular Sciences 23(16): (Journal)
- Registered Authors
- Keywords
- caudal fin, lncRNA, regeneration, vmp1, zebrafish
- Datasets
- GEO:GSE160909
- MeSH Terms
-
- Animals
- In Situ Hybridization
- Wound Healing
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Zebrafish*/metabolism
- RNA, Long Noncoding*/genetics
- RNA, Long Noncoding*/metabolism
- PubMed
- 36012210 Full text @ Int. J. Mol. Sci.
Citation
Li, J., Wen, W., Zhang, S., Zhou, C., Feng, Y., Li, X. (2022) The Expression and Function of lincRNA-154324 and the Adjoining Protein-Coding Gene vmp1 in the Caudal Fin Regeneration of Zebrafish. International Journal of Molecular Sciences. 23(16).
Abstract
Caudal fin regeneration is regulated by a variety of mechanisms, but the role of long non-coding RNA (lncRNA) has rarely been studied. The present study aimed to describe the landscape of lncRNAs during caudal fin regeneration using whole transcriptome sequencing, and then to conduct a functional study on the target lncRNAs using real-time fluorescent quantitative PCR (RT-qPCR), in situ hybridization, and the CRISPR/Cas9 method for lncRNA gene knockout. The results of the transcriptome sequencing showed that a total of 381 lncRNAs were differentially expressed, among which ENSDART00000154324 (lincRNA-154324) was found to be highly related to caudal fin regeneration, and thus it was chosen as the target lncRNA for the subsequent functional study. The results regarding the temporal and spatial expression of lincRNA-154324 and the gene knockout results from CRISPR/Cas9 indicated that lincRNA-154324 is involved in the caudal fin regeneration of zebrafish. Importantly, we serendipitously discovered that the cis correlation coefficient between lincRNA-154324 and its neighboring gene vacuole membrane protein 1 (vmp1) is extremely high, and they are essential for the process of caudal fin regeneration. Moreover, studies have found that vmp1 plays an important role in protein secretion, organelle formation, multicellular development, and autophagy. Collectively, our result may provide a framework for the identification and analysis of lncRNAs involved in the regeneration of the zebrafish caudal fin.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping