PUBLICATION
Silver nanoparticles induce developmental toxicity via oxidative stress and mitochondrial dysfunction in zebrafish (Danio rerio)
- Authors
- Lu, C., Lv, Y., Kou, G., Liu, Y., Liu, Y., Chen, Y., Wu, X., Yang, F., Luo, J., Yang, X.
- ID
- ZDB-PUB-220824-25
- Date
- 2022
- Source
- Ecotoxicology and environmental safety 243: 113993 (Journal)
- Registered Authors
- Chen, Yang, Liu, Yi, Lu, Chunjiao, Luo, Juanjuan, Yang, Xiaojun
- Keywords
- AgNPs, Developmental toxicity, Mitochondrial dysfunction, Oxidative stress, Zebrafish
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian
- Humans
- Metal Nanoparticles*/toxicity
- Mitochondria/metabolism
- Oxidative Stress
- Silver/metabolism
- Superoxide Dismutase/metabolism
- Water Pollutants, Chemical*/metabolism
- Zebrafish/genetics
- Zebrafish/metabolism
- PubMed
- 35994909 Full text @ Ecotoxicol. Environ. Saf.
Citation
Lu, C., Lv, Y., Kou, G., Liu, Y., Liu, Y., Chen, Y., Wu, X., Yang, F., Luo, J., Yang, X. (2022) Silver nanoparticles induce developmental toxicity via oxidative stress and mitochondrial dysfunction in zebrafish (Danio rerio). Ecotoxicology and environmental safety. 243:113993.
Abstract
Sliver nanoparticles (AgNPs) are widely used in industry, agriculture, and medicine, potentially resulting in adverse effects on human health and aquatic environments. Here, we investigated the developmental toxicity of zebrafish embryos with acute exposure to AgNPs. Our results demonstrated developmental defects in 4 hpf zebrafish embryos after exposure to different concentrations of AgNPs for 72 h. In addition, RNA-seq profiling of zebrafish embryos after AgNPs treatment. Further Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses showed that the differentially expressed genes (DEGs) were enriched in DNA replication initiation, oxidoreductase activity, DNA replication, cellular senescence, and oxidative phosphorylation signaling pathways in the AgNPs-treated group. Notably, we also found that AgNPs exposure could result in the accumulation of reactive oxygen species (ROS) and malondialdehyde (MDA), the inhibition of superoxide dismutase (SOD), catalase (CAT), and mitochondrial complex I-V activities, and the downregulated expression of SOD, CAT, and mitochondrial complex I-IV chain-related genes. Moreover, the expression of mitochondrion-mediated apoptosis signaling pathway-related genes, such as bax, bcl2, caspase-3, and caspase-9, was significantly regulated after AgNPs exposure in zebrafish. Therefore, these findings demonstrated that AgNPs exposure could cause oxidative stress, induce mitochondrial dysfunction, and ultimately lead to developmental toxicity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping