PUBLICATION
The Relationship between Procyanidin Structure and Their Protective Effect in a Parkinson's Disease Model
- Authors
- Chen, J., Chen, Y., Zheng, Y., Zhao, J., Yu, H., Zhu, J.
- ID
- ZDB-PUB-220813-7
- Date
- 2022
- Source
- Molecules 27(15): (Journal)
- Registered Authors
- Keywords
- Nrf2/ARE pathway, PC12 cells, Parkinson’s disease, procyanidins, zebrafish
- MeSH Terms
-
- 1-Methyl-4-phenylpyridinium
- Animals
- Biflavonoids
- Catechin
- Oxidative Stress
- Parkinson Disease*/drug therapy
- Proanthocyanidins*/chemistry
- Proanthocyanidins*/pharmacology
- Rats
- Selegiline/pharmacology
- Zebrafish
- PubMed
- 35956957 Full text @ Molecules
Citation
Chen, J., Chen, Y., Zheng, Y., Zhao, J., Yu, H., Zhu, J. (2022) The Relationship between Procyanidin Structure and Their Protective Effect in a Parkinson's Disease Model. Molecules. 27(15).
Abstract
This study evaluated the effect of grape seed-derived monomer, dimeric, and trimeric procyanidins on rat pheochromocytoma cell line (PC12) cells and in a zebrafish Parkinson's disease (PD) model. PC12 cells were cultured with grape seed-derived procyanidins or deprenyl for 24 h and then exposed to 1.5 mm 1-methyl-4-phenylpyridinium (MPP+) for 24 h. Zebrafish larvae (AB strain) 3 days post-fertilization were incubated with deprenyl or grape seed-derived procyanidins in 400 µM 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) for 4 days. The results showed that the procyanidin dimers procyanidin B1 (B1), procyanidin B2 (B2), procyanidin B3 (B3), procyanidin B4 (B4), procyanidin B1-3-O-gallate (B1-G), procyanidin B2-3-O-gallate (B2-G), and the procyanidin trimer procyanidin C1 (C1) had a protective effect on PC12 cells, decreasing the damaged dopaminergic neurons and motor impairment in zebrafish. In PC12 cells and the zebrafish PD model, procyanidin (B1, B2, B3, B4, B1-G, B2-G, C1) treatment decreased the content of reactive oxygen species (ROS) and malondialdehyde (MDA), increased the activity of antioxidant enzymes glutathione peroxidase (GSH-Px), catalase (CAT), and superoxide dismutase (SOD), and upregulated the expression of nuclear factor-erythroid 2-related factor (Nrf2), NAD(P)H: quinone oxidoreductase 1 (NQO1), and heme oxygenase-1 (HO-1). These results suggest that in PC12 cells and the zebrafish PD model, the neuroprotective effects of the procyanidins were positively correlated with their degree of polymerization.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping