PUBLICATION
The combined toxic effects of polyvinyl chloride microplastics and di(2-ethylhexyl) phthalate on the juvenile zebrafish (Danio rerio)
- Authors
- Wang, H., Wang, Y., Wang, Q., Lv, M., Zhao, X., Ji, Y., Han, X., Wang, X., Chen, L.
- ID
- ZDB-PUB-220809-1
- Date
- 2022
- Source
- Journal of hazardous materials 440: 129711 (Journal)
- Registered Authors
- Keywords
- Antagonize toxic effects, Cardiotoxicity effects, Embryonic developmental toxicity, Microplastics, Oxidative stress toxicity
- MeSH Terms
-
- Animals
- Antioxidants/metabolism
- Diethylhexyl Phthalate*/metabolism
- Diethylhexyl Phthalate*/toxicity
- Microplastics/toxicity
- NF-E2-Related Factor 2/metabolism
- Phthalic Acids
- Plastics/metabolism
- Polyvinyl Chloride/toxicity
- Reactive Oxygen Species/metabolism
- Water Pollutants, Chemical*/metabolism
- Water Pollutants, Chemical*/toxicity
- Zebrafish/metabolism
- PubMed
- 35933861 Full text @ J. Hazard. Mater.
Citation
Wang, H., Wang, Y., Wang, Q., Lv, M., Zhao, X., Ji, Y., Han, X., Wang, X., Chen, L. (2022) The combined toxic effects of polyvinyl chloride microplastics and di(2-ethylhexyl) phthalate on the juvenile zebrafish (Danio rerio). Journal of hazardous materials. 440:129711.
Abstract
Microplastics (MPs) have the characteristics of large specific surface area, high hydrophobicity and surface charge, so they are easy to combine with other pollutants and cause toxic effects on aquatic organisms. Here, we prepared a polyvinyl chloride-microplastics (PVC-MPs) fragmentation model to simulate the real microplastic state, and characterized its composition, morphology, particle size and zeta potential. On this basis, we used single and compound exposure of PVC and di(2-ethylhexyl) phthalate (DEHP) to explore their effects on hatchability and mortality of zebrafish (Danio rerio) embryos and toxicity to oxidative stress and cardiac development in zebrafish larvae. Herein, PVC-MPs slowed down the hatching rate of zebrafish embryos and induced the death of zebrafish, while DEHP could slow down the induced of death, it had no effect on hatching rate. The PVC-MPs/DEHP single pollution could induce the reactive oxygen species (ROS) and activated the antioxidant defense signaling pathway, while the compound group showed the level of feedback autoregulation of NF-E2-related factor 2 (Nrf2) signaling pathway. The single pollution also could inhibit the expression of genes related to cardiac development, while the combined pollution showed an antagonistic effect. This study provided a theoretical basis for the ecotoxicology and biomonitoring of MPs in the natural state.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping