PUBLICATION
Myogenic regulatory factors myod and Myf5 are required for dorsal aorta formation and angiogenic sprouting
- Authors
- Paulissen, E., Martin, B.L.
- ID
- ZDB-PUB-220803-5
- Date
- 2022
- Source
- Developmental Biology 490: 134-143 (Journal)
- Registered Authors
- Martin, Benjamin
- Keywords
- Artery, Cardiovascular, Myf5, Myod, Myogenesis, VEGF, Vein, Zebrafish
- MeSH Terms
-
- Animals
- Aorta/metabolism
- Gene Expression Regulation, Developmental
- Hedgehog Proteins/genetics
- Hedgehog Proteins/metabolism
- Muscle Proteins/genetics
- Muscle, Skeletal
- MyoD Protein*/genetics
- MyoD Protein*/metabolism
- Myogenic Regulatory Factor 5/genetics
- Myogenic Regulatory Factor 5/metabolism
- Myogenic Regulatory Factors*/genetics
- Myogenic Regulatory Factors*/metabolism
- Vascular Endothelial Growth Factor A/metabolism
- Zebrafish/genetics
- Zebrafish/metabolism
- PubMed
- 35917935 Full text @ Dev. Biol.
Citation
Paulissen, E., Martin, B.L. (2022) Myogenic regulatory factors myod and Myf5 are required for dorsal aorta formation and angiogenic sprouting. Developmental Biology. 490:134-143.
Abstract
The vertebrate embryonic midline vasculature forms in close proximity to the developing skeletal muscle, which originates in the somites. Angioblasts migrate from bilateral positions along the ventral edge of the somites until they meet at the midline, where they sort and differentiate into the dorsal aorta and the cardinal vein. This migration occurs at that the same time that myoblasts in the somites are beginning to differentiate into skeletal muscle, a process which requires the activity of the basic helix loop helix (bHLH) transcription factors Myod and Myf5. Here we examined vasculature formation in myod and myf5 mutant zebrafish. In the absence of skeletal myogenesis, angioblasts migrate normally to the midline but form only the cardinal vein and not the dorsal aorta. The phenotype is due to the failure to activate vascular endothelial growth factor ligand vegfaa expression in the somites, which in turn is required in the adjacent angioblasts for dorsal aorta specification. Myod and Myf5 cooperate with Hedgehog signaling to activate and later maintain vegfaa expression in the medial somites, which is required for angiogenic sprouting from the dorsal aorta. Our work reveals that the early embryonic skeletal musculature in teleosts evolved to organize the midline vasculature during development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping