PUBLICATION
A robust and tunable system for targeted cell ablation in developing embryos
- Authors
- Labbaf, Z., Petratou, K., Ermlich, L., Backer, W., Tarbashevich, K., Reichman-Fried, M., Luschnig, S., Schulte-Merker, S., Raz, E.
- ID
- ZDB-PUB-220802-15
- Date
- 2022
- Source
- Developmental Cell 57(16): 2026-2040.e5 (Journal)
- Registered Authors
- Ermlich, Laura, Labbaf, Zahra, Raz, Erez, Reichman-Fried, Michal, Schulte-Merker, Stefan, Tarbashevich, Katsiyarina
- Keywords
- cell ablation, neuron, notochord, organogenesis, regeneration, tissue ablation, toxin, vasculature, zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Cell Death
- Cell Differentiation
- Drosophila*
- Zebrafish*/genetics
- PubMed
- 35914525 Full text @ Dev. Cell
Citation
Labbaf, Z., Petratou, K., Ermlich, L., Backer, W., Tarbashevich, K., Reichman-Fried, M., Luschnig, S., Schulte-Merker, S., Raz, E. (2022) A robust and tunable system for targeted cell ablation in developing embryos. Developmental Cell. 57(16):2026-2040.e5.
Abstract
Cell ablation is a key method in the research fields of developmental biology, tissue regeneration, and tissue homeostasis. Eliminating specific cell populations allows for characterizing interactions that control cell differentiation, death, behavior, and spatial organization of cells. Current methodologies for inducing cell death suffer from relatively slow kinetics, making them unsuitable for analyzing rapid events and following primary and immediate consequences of the ablation. To address this, we developed a cell-ablation system that is based on bacterial toxin/anti-toxin proteins and enables rapid and cell-autonomous elimination of specific cell types and organs in zebrafish embryos. A unique feature of this system is that it uses an anti-toxin, which allows for controlling the degree and timing of ablation and the resulting phenotypes. The transgenic zebrafish generated in this work represent a highly efficient tool for cell ablation, and this approach is applicable to other model organisms as demonstrated here for Drosophila.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping