PUBLICATION
PDGFRβ+ cells play a dual role as hematopoietic precursors and niche cells during mouse ontogeny
- Authors
- Sá da Bandeira, D., Kilpatrick, A.M., Marques, M., Gomez-Salazar, M., Ventura, T., Gonzalez, Z.N., Stefancova, D., Rossi, F., Vermeren, M., Vink, C.S., Beltran, M., Henderson, N.C., Jung, B., van der Linden, R., van de Werken, H.J.G., van Ijcken, W.F.J., Betsholtz, C., Forbes, S.J., Cuervo, H., Crisan, M.
- ID
- ZDB-PUB-220722-8
- Date
- 2022
- Source
- Cell Reports 40: 111114 (Journal)
- Registered Authors
- Betsholtz, Christer
- Keywords
- AGM single-cell RNA-sequencing, Bmp4, CP: Developmental biology, CP: Stem cell research, HSPC precursor, MSCs, PDGFRβ, VSMCs, hematopoietic niche, osteogenesis, pericytes
- MeSH Terms
-
- Animals
- Hematopoiesis
- Hematopoietic Stem Cells
- Mesonephros*
- Mice
- Receptor, Platelet-Derived Growth Factor beta
- Stromal Cells
- Zebrafish*
- PubMed
- 35858557 Full text @ Cell Rep.
Citation
Sá da Bandeira, D., Kilpatrick, A.M., Marques, M., Gomez-Salazar, M., Ventura, T., Gonzalez, Z.N., Stefancova, D., Rossi, F., Vermeren, M., Vink, C.S., Beltran, M., Henderson, N.C., Jung, B., van der Linden, R., van de Werken, H.J.G., van Ijcken, W.F.J., Betsholtz, C., Forbes, S.J., Cuervo, H., Crisan, M. (2022) PDGFRβ+ cells play a dual role as hematopoietic precursors and niche cells during mouse ontogeny. Cell Reports. 40:111114.
Abstract
Hematopoietic stem cell (HSC) generation in the aorta-gonad-mesonephros region requires HSC specification signals from the surrounding microenvironment. In zebrafish, PDGF-B/PDGFRβ signaling controls hematopoietic stem/progenitor cell (HSPC) generation and is required in the HSC specification niche. Little is known about murine HSPC specification in vivo and whether PDGF-B/PDGFRβ is involved. Here, we show that PDGFRβ is expressed in distinct perivascular stromal cell layers surrounding the mid-gestation dorsal aorta, and its deletion impairs hematopoiesis. We demonstrate that PDGFRβ+ cells play a dual role in murine hematopoiesis. They act in the aortic niche to support HSPCs, and in addition, PDGFRβ+ embryonic precursors give rise to a subset of HSPCs that persist into adulthood. These findings provide crucial information for the controlled production of HSPCs in vitro.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping