PUBLICATION

Macrophages Break Interneuromast Cell Quiescence by Intervening in the Inhibition of Schwann Cells in the Zebrafish Lateral Line

Authors
Lin, M.J., Lee, C.M., Hsu, W.L., Chen, B.C., Lee, S.J.
ID
ZDB-PUB-220720-12
Date
2022
Source
Frontiers in cell and developmental biology   10: 907863 (Journal)
Registered Authors
Lee, Shyh-Jye
Keywords
Schwann cell, Wnt, interneuromast cell, lateral line, macrophage, regeneration
MeSH Terms
none
PubMed
35846366 Full text @ Front Cell Dev Biol
Abstract
In the zebrafish lateral line system, interneuromast cells (INCs) between neuromasts are kept quiescent by underlying Schwann cells (SWCs). Upon severe injuries that cause the complete loss of an entire neuromast, INCs can occasionally differentiate into neuromasts but how they escape from the inhibition by SWCs is still unclear. Using a genetic/chemical method to ablate a neuromast precisely, we found that a small portion of larvae can regenerate a new neuromast. However, the residual regeneration capacity was hindered by inhibiting macrophages. Using in toto imaging, we further discovered heterogeneities in macrophage behavior and distribution along the lateral line. We witnessed the crawling of macrophages between the injured lateral line and SWCs during regeneration and between the second primordium and the first mature lateral line during development. It implies that macrophages may physically alleviate the nerve inhibition to break the dormancy of INCs during regeneration and development in the zebrafish lateral line.
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Allele Construct Type Affected Genomic Region
gl23TgTransgenic Insertion
    i114TgTransgenic Insertion
      kyu1TgTransgenic Insertion
        ru1011TgTransgenic Insertion
          twu201TgTransgenic Insertion
            twu202TgTransgenic Insertion
              zf15TgTransgenic Insertion
                zf106TgTransgenic Insertion
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                  Engineered Foreign Genes
                  Marker Marker Type Name
                  d2EGFPEFGd2EGFP
                  EGFPEFGEGFP
                  GFPEFGGFP
                  KikGREFGKikGR
                  mCherryEFGmCherry
                  NTREFGNTR
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                  Citation
                  Lin, M.J., Lee, C.M., Hsu, W.L., Chen, B.C., Lee, S.J. (2022) Macrophages Break Interneuromast Cell Quiescence by Intervening in the Inhibition of Schwann Cells in the Zebrafish Lateral Line. Frontiers in cell and developmental biology. 10:907863.