PUBLICATION

Gene expression and functional analysis of Aha1a and Aha1b in stress response in zebrafish

Authors
Xiao, H., Wang, H., He, Q., Zhou, J., Du, S.
ID
ZDB-PUB-220714-11
Date
2022
Source
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology   262: 110777 (Journal)
Registered Authors
Du, Shao Jun (Jim), Zhou, Jianfeng
Keywords
Aha1(activator of Hsp90 ATPase), Heat shock protein, Heat shock response, Myosin chaperone
MeSH Terms
  • Adenosine Triphosphatases/metabolism
  • Animals
  • Embryo, Nonmammalian/metabolism*
  • Gene Expression
  • HSP90 Heat-Shock Proteins/metabolism
  • Heat-Shock Response
  • Molecular Chaperones/metabolism*
  • Zebrafish*/genetics
  • Zebrafish*/metabolism
  • Zebrafish Proteins/metabolism*
PubMed
35830921 Full text @ Comp. Biochem. Physiol. B Biochem. Mol. Biol.
Abstract
Activator of heat shock protein 90 (hsp90) ATPase (Aha1) is a Hsp90 co-chaperone required for Hsp90 ATPase activation. Aha1 is essential for yeast survival and muscle development in C. elegans under elevated temperature and hsp90-deficeiency induced stress conditions. The roles of Aha1 in vertebrates are poorly understood. Here, we characterized the expression and function of Aha1 in zebrafish. We showed that zebrafish genome contains two aha1 genes, aha1a and aha1b, that show distinct patterns of expression during development. Under normal the physiological condition, aha1a is primarily expressed in skeletal muscle cells of zebrafish embryos, while aha1b is strongly expressed in the head region. aha1a and aha1b expression increased dramatically in response to heat shock induced stress. In addition, Aha1a-GFP fusion protein exhibited a dynamic translocation in muscle cells in response to heat shock. Moreover, upregulation of aha1 expression was also observed in hsp90a1 knockdown embryos that showed a muscle defect. Genetic studies demonstrated that knockout of aha1a, aha1b or both had no detectable effect on embryonic development, survival, and growth in zebrafish. The aha1a and aha1b mutant embryos showed normal muscle development and stress response in response to heat shock. Single or double aha1a and aha1b mutants could grow into normal reproductive adults with normal skeletal muscle structure and morphology compared with wild type control. Together, data from these studies indicate that Aha1a and Aha1b are involved in stress response. However, they are dispensable in zebrafish embryonic development, growth, and survival.
Genes / Markers
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Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
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Mapping