PUBLICATION
Development a High Throughput Zebrafish Embryo acute toxicity Testing Method based on OECD TG 236
- Authors
- Xu, S., Chen, F., Zhang, H., Huang, Z.L., Li, J., Wu, D., Chen, X.
- ID
- ZDB-PUB-220709-3
- Date
- 2022
- Source
- Toxicology mechanisms and methods 33(2): 104-112 (Journal)
- Registered Authors
- Chen, Xueping, Xu, Shisan
- Keywords
- OECD 236, Zebrafish embryo, acute toxicity, comparison, throughput
- MeSH Terms
-
- Animals
- Biological Assay
- Lethal Dose 50
- Organisation for Economic Co-Operation and Development
- Toxicity Tests, Acute/methods
- Water Pollutants, Chemical*/toxicity
- Zebrafish*
- PubMed
- 35799369 Full text @ Toxicol. Mech. Methods
Citation
Xu, S., Chen, F., Zhang, H., Huang, Z.L., Li, J., Wu, D., Chen, X. (2022) Development a High Throughput Zebrafish Embryo acute toxicity Testing Method based on OECD TG 236. Toxicology mechanisms and methods. 33(2):104-112.
Abstract
The Organization for Economic Co-operation and Development (OECD)Test Guideline (TG) 236 for zebrafish embryo acute toxicity testing was adopted for chemical toxicity assessment in 2013. Due to the increasing demand for prediction and evaluation of the acute toxicity using zebrafish embryos, we developed a method based on OECD 236 test guideline with the aim to improve the testing efficiency. We used 4-128 cell stage zebrafish embryos, and performed an exposure assay in a 96-well microtiter plate, observing the lethality endpoints of embryos at 48 h post exposure. A total of 32 chemicals (two batches) were used in the comparison study. Our results indicated that the logarithmic LC50 (half lethal concentration) obtained by the modified method exhibited good correlation with that obtained by the OECD 236 testing method, and the R2 of the linear regression analysis was 0.9717 (0.9621 and 0.9936 for the two batches, respectively). Additionally, the intra- and inter-laboratory coefficient of variation (CVs) for the LC50 from the testing chemicals (17 chemicals in second batch) was less than 30%, except for CuSO4. Therefore, the developed method was less time-consuming and demonstrated a higher throughput for toxicity testing compared to the prior method. We argue the developed method could be used as an additional choice for high throughput zebrafish embryo acute toxicity test.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping