PUBLICATION
Embryonic nutritional hyperglycemia decreases cell proliferation in the zebrafish retina
- Authors
- Hernández-Núñez, I., Vivero-Lopez, M., Quelle-Regaldie, A., DeGrip, W.J., Sánchez, L., Concheiro, A., Alvarez-Lorenzo, C., Candal, E., Barreiro-Iglesias, A.
- ID
- ZDB-PUB-220703-2
- Date
- 2022
- Source
- Histochemistry and cell biology 158(4): 401-409 (Journal)
- Registered Authors
- Keywords
- Cell proliferation, Diabetic retinopathy, Hyperglycemia, Mitosis, Retina, Zebrafish
- MeSH Terms
-
- Animals
- Cell Proliferation
- Glucose/metabolism
- Hyperglycemia*/metabolism
- Proliferating Cell Nuclear Antigen/metabolism
- Proliferating Cell Nuclear Antigen/pharmacology
- Retina/metabolism
- Zebrafish*
- PubMed
- 35779079 Full text @ Histochem. Cell Biol.
Citation
Hernández-Núñez, I., Vivero-Lopez, M., Quelle-Regaldie, A., DeGrip, W.J., Sánchez, L., Concheiro, A., Alvarez-Lorenzo, C., Candal, E., Barreiro-Iglesias, A. (2022) Embryonic nutritional hyperglycemia decreases cell proliferation in the zebrafish retina. Histochemistry and cell biology. 158(4):401-409.
Abstract
Diabetic retinopathy (DR) is one of the leading causes of blindness in the world. While there is a major focus on the study of juvenile/adult DR, the effects of hyperglycemia during early retinal development are less well studied. Recent studies in embryonic zebrafish models of nutritional hyperglycemia (high-glucose exposure) have revealed that hyperglycemia leads to decreased cell numbers of mature retinal cell types, which has been related to a modest increase in apoptotic cell death and altered cell differentiation. However, how embryonic hyperglycemia impacts cell proliferation in developing retinas still remains unknown. Here, we exposed zebrafish embryos to 50 mM glucose from 10 h postfertilization (hpf) to 5 days postfertilization (dpf). First, we confirmed that hyperglycemia increases apoptotic death and decreases the rod and Müller glia population in the retina of 5-dpf zebrafish. Interestingly, the increase in cell death was mainly observed in the ciliary marginal zone (CMZ), where most of the proliferating cells are located. To analyze the impact of hyperglycemia in cell proliferation, mitotic activity was first quantified using pH3 immunolabeling, which revealed a significant decrease in mitotic cells in the retina (mainly in the CMZ) at 5 dpf. A significant decrease in cell proliferation in the outer nuclear and ganglion cell layers of the central retina in hyperglycemic animals was also detected using the proliferation marker PCNA. Overall, our results show that nutritional hyperglycemia decreases cellular proliferation in the developing retina, which could significantly contribute to the decline in the number of mature retinal cells.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping