PUBLICATION

Induction of endoplasmic reticulum stress markers in an acromegaly model

Authors
Elbialy, A., Sulidan, K., Bhuiyan, A., Igarashi, Y., Yoshitake, K., Yamanouchi, D., Kang, J., Asakawa, S., Watabe, S., Kinoshita, S.
ID
ZDB-PUB-220624-5
Date
2022
Source
Journal of cellular biochemistry   123(9): 1411-1421 (Journal)
Registered Authors
Kang, Junsu, Kinoshita, Shigeharu, Watabe, Shugo
Keywords
XBP1, acromegaly, endoplasmic reticulum (ER) stress, growth hormone, zebrafish
MeSH Terms
  • Aged
  • X-Box Binding Protein 1/genetics
  • Growth Hormone
  • RNA, Messenger/genetics
  • Endoplasmic Reticulum Stress*/genetics
  • Humans
  • Zebrafish/genetics
  • Biomarkers
  • Acromegaly*/genetics
  • Animals
(all 10)
PubMed
35734917 Full text @ J. Cell. Biochem.
Abstract
Acromegaly is a growth hormone (GH) excess pathological condition in humans. Acromegaly is associated with somatic disfigurement and a wide range of systemic manifestations such as arthritis, neuropathy, carpal tunnel syndrome, reproductive disorders, metabolic disorders, and gastrointestinal complications. The influence of excess GH on the cellular level could aid in understanding the root causes of acromegaly-related health complications. Previously, we found that GH excess induces DNA damage to somatic cells and reduces the stem cells number and causes premature aging. In this study, an in-depth analysis of the acromegaly RNAseq data revealed the disruption of important biological cellular processes. Gene set enrichment analysis, heatmap, and enrichment analysis of acromegaly RNAseq data revealed induction of endoplasmic reticulum (ER) stress markers in various organs. Interestingly, the induction of ER stress was even more apparent than in aged zebrafish. Splicing of box-binding protein-1 (XBP1) mRNA is a hallmark of ER stress. Therefore, we quantified spliced XBP1 mRNA in different organs of our acromegaly model. Thus, our study emphasizes the importance of ER stress in GH oversecretion, which is important for understanding the health complications of acromegaly.
Genes / Markers
Figures
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
kj39Tgstandard conditionsPrim-5
kj39Tgstandard conditionsProtruding-mouth
kj39Tgstandard conditionsDay 5
kj39Tgstandard conditionsAdult
kj39Tgstandard conditionsAdult
kj39Tgstandard conditionsAdult
kj39Tgstandard conditionsAdult
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
kj39TgTransgenic Insertion
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    Human Disease / Model
    Human Disease Fish Conditions Evidence
    acromegalykj39Tgstandard conditionsTAS
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    Sequence Targeting Reagents
    No data available
    Fish
    Fish
    kj39Tg
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    Antibodies
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    Orthology
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    Engineered Foreign Genes
    Marker Marker Type Name
    DsRed2EFGDsRed2
    1 - 1 of 1
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    Mapping
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    Citation
    Elbialy, A., Sulidan, K., Bhuiyan, A., Igarashi, Y., Yoshitake, K., Yamanouchi, D., Kang, J., Asakawa, S., Watabe, S., Kinoshita, S. (2022) Induction of endoplasmic reticulum stress markers in an acromegaly model. Journal of cellular biochemistry. 123(9):1411-1421.