PUBLICATION
Blood Flow Regulates Glomerular Capillary Formation in Zebrafish Pronephros
- Authors
- Nishimura, Y., Ishii, T., Ando, K., Yuge, S., Nakajima, H., Zhou, W., Mochizuki, N., Fukuhara, S.
- ID
- ZDB-PUB-220622-52
- Date
- 2022
- Source
- Kidney360 3: 700-713 (Journal)
- Registered Authors
- Ando, Koji, Ishii, Tomohiro, Mochizuki, Naoki, Nakajima, Hiroyuki, Yuge, Shinya, Zhou, Weibin
- Keywords
- basic science, cardiovascular, cell biology and structure, genetics and development, glomerular endothelial cells, glomerulus, hemodynamics, hemodynamics and vascular regulation, organogenesis, pronephros, vascular endothelial growth factor, zebrafish
- MeSH Terms
-
- Animals
- Endothelial Cells
- Kidney Glomerulus/blood supply
- Pronephros*/metabolism
- Vascular Endothelial Growth Factor A/metabolism
- Zebrafish*
- PubMed
- 35721616 Full text @ Kidney360
Citation
Nishimura, Y., Ishii, T., Ando, K., Yuge, S., Nakajima, H., Zhou, W., Mochizuki, N., Fukuhara, S. (2022) Blood Flow Regulates Glomerular Capillary Formation in Zebrafish Pronephros. Kidney360. 3:700-713.
Abstract
Background The renal glomerulus is a tuft of capillaries in Bowman's capsule and functions as a blood-filtration unit in the kidney. The unique glomerular capillary tuft structure is relatively conserved through vertebrate species. However, the morphogenetic mechanism governing glomerular capillary tuft formation remains elusive.
Methods To clarify how glomerular capillaries develop, we analyzed glomerular capillary formation in the zebrafish pronephros by exploiting fluorescence-based bio-imaging technology.
Results During glomerular capillary formation in the zebrafish pronephros, endothelial cells initially sprouted from the dorsal aorta and formed the capillaries surrounding the bilateral glomerular primordia in response to podocyte progenitor-derived vascular endothelial growth factor-A. After formation, blood flow immediately occurred in the glomerular primordia-associated capillaries, while in the absence of blood flow, they were transformed into sheet-like structures enveloping the glomerular primordia. Subsequently, blood flow induced formation of Bowman's space at the lateral sides of the bilateral glomerular primordia. Concomitantly, podocyte progenitors enveloped their surrounding capillaries while moving toward and coalescing at the midline. These capillaries then underwent extensive expansion and remodeling to establish a functional glomerular capillary tuft. However, stopping blood flow inhibited the remodeling of bilateral glomerular primordia, which therefore remained unvascularized but covered by the vascular sheets.
Conclusions We delineated the morphogenetic processes governing glomerular capillary tuft formation in the zebrafish pronephros and demonstrated crucial roles of blood flow in its formation. Blood flow maintains tubular structures of the capillaries surrounding the glomerular primordia and promotes glomerular incorporation of these vessels by inducing the remodeling of glomerular primordia.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping