PUBLICATION
Assessment of resveratrol on diabetes of zebrafish (Danio rerio)
- Authors
- Faal, M., Manouchehri, H., Changizi, R., Bootorabi, F., Khorramizadeh, M.R.
- ID
- ZDB-PUB-220609-3
- Date
- 2022
- Source
- Journal of diabetes and metabolic disorders 21: 823-833 (Journal)
- Registered Authors
- Bootorabi, Fatemeh, Changizi, Reza, Faal, Mina, Khorramizadeh, M.Reza
- Keywords
- Anti-inflammation, Diabetes mellitus, Gene expression, Hyperglycemia, Resveratrol, Zebrafish
- MeSH Terms
- none
- PubMed
- 35673499 Full text @ J Diabetes Metab Disord
Citation
Faal, M., Manouchehri, H., Changizi, R., Bootorabi, F., Khorramizadeh, M.R. (2022) Assessment of resveratrol on diabetes of zebrafish (Danio rerio). Journal of diabetes and metabolic disorders. 21:823-833.
Abstract
Purpose Zebrafish (Danio rerio) is an established model for studying various metabolic diseases. The aim of this study was to evaluate the effect of resveratrol as a natural polyphenol on reducing inflammation caused by hyperglycemia (diabetes) and its effect on digestive tissue as well as TNF-α, IFN-γ, and INL1β genes in zebrafish.
Methods Within a 20-day period, the research was performed on 120 adult zebrafish, which were randomly classified into eight groups: two experimental treatments (induced glucose = +G) and (without glucose = -G), where each main group was as follows: CTRL = control and RSV resveratrol with doses 10, 20, and 30 μmol/L. At the end of the period, the blood glucose level was measured using glucose test strip, staining of intestinal tissue was done by hematoxylin and eosin (H&E), and expression of INF-γ, IL1-β, and TNF-α genes extracted from the intestinal was measured via internal method RT-PCR. Data analysis in this study was performed using SPSS software version 21. One-way ANOVA and mean comparison of treatments by Duncan test were used for data analysis. All statistical analyses were performed at a significant level (P < 0.5) where the mean data were presented with standard deviation.
Results According to the results, the lowest blood sugar level at the end of the experiment belonged to the group (G-RSV20) where no significant difference was observed between treatments (P > 0.05). The highest expression of IL1-β gene belonged to the (G + CTRL) group (P < 0.05), while the (G + RSV20) group showed the lowest expression of the INF-γ gene and had a significant difference with other groups (P < 0.05). In (G + RSV10) treatment, the lowest expression of TNF-α gene was observed and there was no significant difference with other treatments (P > 0.05). Resveratrol would improve the absorption of nutrients in the intestinal tissue by increasing the number of goblet cells as well as the width and height of the villi.
Conclusion Collectively, this study indicated that treatment with resveratrol could improve metabolic-mediated performances by reducing blood glucose, increasing food absorption in the intestine tissue, and reducing the expression of inflammatory genes in type 2 diabetic zebrafish model.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping