PUBLICATION
Simple and High-Throughput Rheotaxis Behavioral Assay for Zebrafish Larva
- Authors
- Tantry, M.S.A., Harini, V.S., Santhakumar, K.
- ID
- ZDB-PUB-220607-25
- Date
- 2022
- Source
- Zebrafish 19(3): 114-118 (Journal)
- Registered Authors
- Santhakumar, Kiran
- Keywords
- behavior assay, copper sulfate, lateral line, ototoxicity, rheotaxis, zebrafish
- MeSH Terms
-
- Animals
- Behavior, Animal
- Hair Cells, Auditory*
- High-Throughput Screening Assays/methods
- Larva
- Zebrafish*
- PubMed
- 35666213 Full text @ Zebrafish
Citation
Tantry, M.S.A., Harini, V.S., Santhakumar, K. (2022) Simple and High-Throughput Rheotaxis Behavioral Assay for Zebrafish Larva. Zebrafish. 19(3):114-118.
Abstract
Zebrafish (Danio rerio) is used as a model for studying sensorineural hearing loss. The damage to the hair cells can be assessed by scoring rheotaxis behavior in zebrafish. In this study, we newly designed a rheotaxis behavioral assay protocol capable of quantifying rheotaxis behavior in zebrafish larvae. We chemically induced ototoxicity in the larvae using copper sulfate, a well-known ototoxin, and determined rheotaxis at different flow velocities. The simple, cost-effective, and high-throughput rheotaxis assay system can provide great insights into drug development and other behavioral studies.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping