PUBLICATION
A New Transgenic Line for Rapid and Complete Neutrophil Ablation
- Authors
- Hall, C.J., Astin, J.W., Mumm, J.S., Ackerley, D.F.
- ID
- ZDB-PUB-220527-16
- Date
- 2022
- Source
- Zebrafish 19(3): 109-113 (Journal)
- Registered Authors
- Astin, Jonathan, Hall, Chris, Mumm, Jeff
- Keywords
- NTR 2.0, ablation, metronidazole, neutrophil, nitroreductase
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Metronidazole/pharmacology
- Neutrophils*/metabolism
- Nitroreductases/genetics
- Nitroreductases/metabolism
- Zebrafish*/physiology
- PubMed
- 35617702 Full text @ Zebrafish
Citation
Hall, C.J., Astin, J.W., Mumm, J.S., Ackerley, D.F. (2022) A New Transgenic Line for Rapid and Complete Neutrophil Ablation. Zebrafish. 19(3):109-113.
Abstract
Zebrafish lines expressing nitroreductase (NTR) in specific cell compartments, which sensitizes those cells to metronidazole (MTZ)-mediated ablation, have proven extremely useful for studying tissue regeneration and investigating cell function. In contrast to many cells, neutrophils are comparatively resistant to the NTR/MTZ targeted ablation strategy. Recently, a rationally engineered variant of NTR (NTR 2.0) has been described that exhibits greatly improved MTZ-mediated ablation efficacy in zebrafish. We show that a transgenic line with neutrophil-restricted expression of NTR 2.0 demonstrates complete neutrophil ablation, with an MTZ dose 100-fold less than current treatment regimens, and with treatment durations as short as 5 h.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping