PUBLICATION
Pericentriolar matrix (PCM) integrity relies on cenexin and Polo-Like Kinase (PLK)1
- Authors
- Aljiboury, A., Mujcic, A., Curtis, E., Cammerino, T., Magny, D., Lan, Y., Bates, M., Freshour, J., Ahmed-Braimeh, Y.H., Hehnly, H.
- ID
- ZDB-PUB-220525-4
- Date
- 2022
- Source
- Molecular biology of the cell 33(9): br14 (Journal)
- Registered Authors
- Hehnly, Heidi
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Cycle Proteins*/metabolism
- Centrosome*/metabolism
- HeLa Cells
- Heat-Shock Proteins*/genetics
- Heat-Shock Proteins*/metabolism
- Humans
- Microtubules/metabolism
- Phosphorylation
- Protein Binding
- Protein Serine-Threonine Kinases*/metabolism
- Proto-Oncogene Proteins*/metabolism
- Zebrafish*/embryology
- Zebrafish*/genetics
- Zebrafish*/metabolism
- Zebrafish Proteins*/deficiency
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- PubMed
- 35609215 Full text @ Mol. Biol. Cell
Citation
Aljiboury, A., Mujcic, A., Curtis, E., Cammerino, T., Magny, D., Lan, Y., Bates, M., Freshour, J., Ahmed-Braimeh, Y.H., Hehnly, H. (2022) Pericentriolar matrix (PCM) integrity relies on cenexin and Polo-Like Kinase (PLK)1. Molecular biology of the cell. 33(9):br14.
Abstract
Polo-Like-Kinase (PLK) 1 activity is associated with maintaining the functional and physical properties of the centrosome's pericentriolar matrix (PCM). In this study, we use a multimodal approach of human cells (HeLa), zebrafish embryos, and phylogenic analysis to test the role of a PLK1 binding protein, cenexin, in regulating the PCM. Our studies identify that cenexin is required for tempering microtubule nucleation by maintaining PCM cohesion in a PLK1 dependent manner. PCM architecture in cenexin-depleted zebrafish embryos was rescued with wild-type human cenexin, but not with a C-terminal cenexin mutant (S796A) deficient in PLK1 binding. We propose a model where cenexin's C-terminus acts in a conserved manner in eukaryotes, excluding nematodes and arthropods, to sequester PLK1 that limits PCM substrate phosphorylation events required for PCM cohesion. [Media: see text].
Errata / Notes
Article Link: https://www.molbiolcell.org/doi/full/10.1091/mbc.E22-01-0015
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping