PUBLICATION

Single-cell transcriptome reveals insights into the development and function of the zebrafish ovary

Authors
Liu, Y., Kassack, M.E., McFaul, M.E., Christensen, L.N., Siebert, S., Wyatt, S.R., Kamei, C.N., Horst, S., Arroyo, N., Drummond, I.A., Juliano, C.E., Draper, B.W.
ID
ZDB-PUB-220520-8
Date
2022
Source
eLIFE   11: (Journal)
Registered Authors
Draper, Bruce, Drummond, Iain, Liu, Yulong, McFaul, Matthew, Wyatt, Sydney
Keywords
developmental biology, zebrafish
Datasets
GEO:GSE191137
MeSH Terms
  • Female
  • Ovary*/metabolism
  • Zebrafish*/genetics
  • Gonads
  • Transcriptome
  • Animals
  • Sex Differentiation/genetics
(all 7)
PubMed
35588359 Full text @ Elife
Abstract
Zebrafish are an established research organism that has made many contributions to our understanding of vertebrate tissue and organ development, yet there are still significant gaps in our understanding of the genes that regulate gonad development, sex, and reproduction. Unlike the development of many organs, such as the brain and heart that form during the first few days of development, zebrafish gonads do not begin to form until the larval stage (≥5 dpf). Thus, forward genetic screens have identified very few genes required for gonad development. In addition, bulk RNA sequencing studies which identify genes expressed in the gonads do not have the resolution necessary to define minor cell populations that may play significant roles in development and function of these organs. To overcome these limitations, we have used single-cell RNA sequencing to determine the transcriptomes of cells isolated from juvenile zebrafish ovaries. This resulted in the profiles of 10,658 germ cells and 14,431 somatic cells. Our germ cell data represents all developmental stages from germline stem cells to early meiotic oocytes. Our somatic cell data represents all known somatic cell types, including follicle cells, theca cells and ovarian stromal cells. Further analysis revealed an unexpected number of cell subpopulations within these broadly defined cell types. To further define their functional significance, we determined the location of these cell subpopulations within the ovary. Finally, we used gene knockout experiments to determine the roles of foxl2l and wnt9b for oocyte development and sex determination and/or differentiation, respectively. Our results reveal novel insights into zebrafish ovarian development and function and the transcriptome profiles will provide a valuable resource for future studies.
Genes / Markers
Figures
Figure Gallery (11 images) / 2
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ca7TgTransgenic Insertion
    fb207
      Small Deletion
        fb209
          Small Deletion
          pd27TgTransgenic Insertion
            uc02TgTransgenic Insertion
              uc91TgTransgenic Insertion
              y1TgTransgenic Insertion
                1 - 7 of 7
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                Human Disease / Model
                No data available
                Sequence Targeting Reagents
                Target Reagent Reagent Type
                foxl2lCRISPR1-foxl2lCRISPR
                wnt9bCRISPR1-wnt9bCRISPR
                1 - 2 of 2
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                Fish
                Antibodies
                Name Type Antigen Genes Isotypes Host Organism
                Ab1-ddx4Rabbit
                Ab5-ddx4polyclonal
                  IgYChicken
                  1 - 2 of 2
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                  Orthology
                  No data available
                  Engineered Foreign Genes
                  Marker Marker Type Name
                  DsRed2EFGDsRed2
                  EGFPEFGEGFP
                  1 - 2 of 2
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                  Mapping
                  No data available