PUBLICATION
Structural characterization of a mannoglucan polysaccharide from Dendrobium huoshanense and evaluation of its osteogenesis promotion activities
- Authors
- Li, X., Zhang, Q., Zhu, Y., Li, Y., Mei, S., Luo, H., Wu, K.
- ID
- ZDB-PUB-220517-26
- Date
- 2022
- Source
- International journal of biological macromolecules 211: 441-449 (Journal)
- Registered Authors
- Keywords
- Dendrobium huoshanense, Eyzymatic analysis, Mannoglucan, NMR, Osteogenesis promotion
- MeSH Terms
-
- Animals
- Dendrobium*/chemistry
- Dietary Carbohydrates
- Osteogenesis
- Polysaccharides/chemistry
- Zebrafish
- PubMed
- 35577191 Full text @ Int. J. Biol. Macromol.
Citation
Li, X., Zhang, Q., Zhu, Y., Li, Y., Mei, S., Luo, H., Wu, K. (2022) Structural characterization of a mannoglucan polysaccharide from Dendrobium huoshanense and evaluation of its osteogenesis promotion activities. International journal of biological macromolecules. 211:441-449.
Abstract
Dendrobium huoshanense, a valuable traditional Chinese herb, is widely used to prolong life in China. Our study aims to characterize the structure and osteogenesis-promotion effects of a heteropolysaccharide component isolated from Dendrobium huoshanense (DHPW1). The structure of DHPW1 was characterized using gas chromatography-mass spectrometry and nuclear magnetic resonance, while its osteogenic activity was evaluated using MG-63 cells and zebrafish skulls. The results showed that the molecular weight of DHPW1 was 230 kDa and it was mainly composed of mannose and glucose. In addition, the DHPW1 backbone consisted of (1 → 4)-linked-β-D-Mannopyranosyl and (1 → 4)-linked-β-d-Glucopyranosyl. Furthermore, DHPW1 significantly increased ALP activity and mineralized nodule formation in MG-63 cells. DHPW1 in zebrafish skull models significantly enhanced the relative fluorescence intensity of bone mass and increased the degree of bone mineralization. These results suggested that the DHPW1 component in D. huoshanense has potential to promote osteogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping