PUBLICATION
Zebrafish Larvae Rapidly Recover from Locomotor Effects and Neuromuscular Alterations Induced by Cholinergic Insecticides
- Authors
- Könemann, S., von Wyl, M., Vom Berg, C.
- ID
- ZDB-PUB-220517-19
- Date
- 2022
- Source
- Environmental science & technology 56(12): 8449-8462 (Journal)
- Registered Authors
- vom Berg, Colette
- Keywords
- axon growth, birefringence, immunohistochemistry, locomotion, muscle development, neuromuscular junction, recovery
- MeSH Terms
-
- Animals
- Cholinergic Agents/pharmacology
- Insecticides*/toxicity
- Larva
- Methomyl/pharmacology
- Neonicotinoids
- Zebrafish
- PubMed
- 35575681 Full text @ Env. Sci. Tech.
Citation
Könemann, S., von Wyl, M., Vom Berg, C. (2022) Zebrafish Larvae Rapidly Recover from Locomotor Effects and Neuromuscular Alterations Induced by Cholinergic Insecticides. Environmental science & technology. 56(12):8449-8462.
Abstract
Owing to the importance of acetylcholine as a neurotransmitter, many insecticides target the cholinergic system. Across phyla, cholinergic signaling is essential for many neuro-developmental processes including axonal pathfinding and synaptogenesis. Consequently, early-life exposure to such insecticides can disturb these processes, resulting in an impaired nervous system. One test frequently used to assess developmental neurotoxicity is the zebrafish light-dark transition test, which measures larval locomotion as a response to light changes. However, it is only poorly understood which structural alterations cause insecticide-induced locomotion defects and how persistent these alterations are. Therefore, this study aimed to link locomotion defects with effects on neuromuscular structures, including motorneurons, synapses, and muscles, and to investigate the longevity of the effects. The cholinergic insecticides diazinon and dimethoate (organophosphates), methomyl and pirimicarb (carbamates), and imidacloprid and thiacloprid (neonicotinoids) were used to induce hypoactivity. Our analyses revealed that some insecticides did not alter any of the structures assessed, while others affected axon branching (methomyl, imidacloprid) or muscle integrity (methomyl, thiacloprid). The majority of effects, even structural, were reversible within 24 to 72 h. Overall, we find that both neurodevelopmental and non-neurodevelopmental effects of different longevity can account for the reduced locomotion. These findings provide unprecedented insights into the underpinnings of insecticide-induced hypoactivity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping