PUBLICATION
3β-Hydroxy-Δ5-steroidal congeners from a column fraction of Dendronephthya puetteri attenuate LPS-induced inflammatory responses in RAW 264.7 macrophages and zebrafish embryo model
- Authors
- Fernando, I.P.S., Lee, W.W., Jayawardena, T.U., Kang, M.C., Ann, Y.S., Ko, C.I., Park, Y.J., Jeon, Y.J.
- ID
- ZDB-PUB-220512-9
- Date
- 2018
- Source
- RSC Advances 8: 18626-18634 (Journal)
- Registered Authors
- Jeon, You-Jin
- Keywords
- none
- MeSH Terms
- none
- PubMed
- 35541104 Full text @ RSC Adv.
Citation
Fernando, I.P.S., Lee, W.W., Jayawardena, T.U., Kang, M.C., Ann, Y.S., Ko, C.I., Park, Y.J., Jeon, Y.J. (2018) 3β-Hydroxy-Δ5-steroidal congeners from a column fraction of Dendronephthya puetteri attenuate LPS-induced inflammatory responses in RAW 264.7 macrophages and zebrafish embryo model. RSC Advances. 8:18626-18634.
Abstract
Bioactive compounds from marine organisms and their action mechanisms have provided new insights into medicinal and natural product research. Here, we report the identification of 3β-hydroxy-Δ5-steroidal congeners from a purified column fraction (DPCMH24) of the soft coral Dendronephthya puetteri harvested from Jeju, South Korea. DPCMH24 exerted strong anti-inflammatory effects through a dose-dependent decrease in the levels of nitric oxide (NO) in LPS-induced RAW 264.7 macrophages (IC50 value = 6.54 ± 0.38 μg mL-1). Further, DPCMH24 attenuated the levels of PGE2 and the pro-inflammatory cytokines, TNF-α, IL-1β, and IL-6. The above effects were mediated via the inhibition of nuclear factor κB activation and mitogen-activated protein kinase pathways. In vivo evaluation indicated that DPCMH24 reduced NO, iNOS, COX-2, ROS production and cell death in LPS-induced zebrafish embryos, confirming its anti-inflammatory potential. The constituent compounds were identified by GC-MS/MS analysis. These findings suggest that the steroidal congeners from D. puetteri may offer ample therapeutic potential against LPS-induced inflammation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping