PUBLICATION
Transcriptome analysis of developing zebrafish (Danio rerio) embryo following exposure to Gaudichaudione H reveals teratogenicity and cardiovascular defects caused by abnormal iron metabolism
- Authors
- Gao, X.M., Li, B., Wang, M.Y., Liu, H.D., Tang, L.P., Wang, F., Yan, D.M., Han, X.Y., Xu, L.X.
- ID
- ZDB-PUB-220508-5
- Date
- 2022
- Source
- Chemico-biological interactions 361: 109968 (Journal)
- Registered Authors
- Wang, Mingyong
- Keywords
- Caged xanthone, Cardiovascular toxicity, Gaudichaudione H, Iron metabolism, Teratogenicity, Zebrafish
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian
- Gene Expression Profiling
- Iron/metabolism
- Larva
- Teratogenesis*
- Water Pollutants, Chemical*/toxicity
- Zebrafish
- PubMed
- 35525316 Full text @ Chem. Biol. Interact.
Citation
Gao, X.M., Li, B., Wang, M.Y., Liu, H.D., Tang, L.P., Wang, F., Yan, D.M., Han, X.Y., Xu, L.X. (2022) Transcriptome analysis of developing zebrafish (Danio rerio) embryo following exposure to Gaudichaudione H reveals teratogenicity and cardiovascular defects caused by abnormal iron metabolism. Chemico-biological interactions. 361:109968.
Abstract
Gaudichaudione H (GH), a caged polyprenylated xanthone from Garcinia plants, showed anti-cancer and anti-inflammatory effects in vitro. However, the in vivo toxicity of this compound has never been reported. The present study was aimed to address the toxic effects of Gaudichaudione H using zebrafish embryos and larvae as an in vivo test model. The zebrafish embryos were treated with GH having different concentrations (0, 0.28, 0.38 and 0.57 μg/mL). The results revealed that GH induces significant embryonic mortality, decreased heartbeat, cardiotoxicity, cardiovascular defects, increased apoptosis and decreased hemoglobinization in zebrafish embryos and larvae. According to transcriptome analysis, 1841 genes were significantly differentially expressed (1185 down-regulated and 656 up-regulated) after GH treatment. The main functions of these genes were related to iron metabolism pathways. The toxicity of GH on zebrafish embryonic development and cardiovascular may due to large amounts of downregulated genes involved in metabolic pathways and DEGs related to 'Iron ion binding' and 'Heme binding' functions.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping