PUBLICATION

Abnormal male reproduction and embryonic development induced by downregulation of a phospholipid fatty acid-introducing enzyme Lpgat1 in zebrafish

Authors
Shibata, T., Kawana, H., Nishino, Y., Ito, Y., Sato, H., Onishi, H., Kano, K., Inoue, A., Taketomi, Y., Murakami, M., Kofuji, S., Nishina, H., Miyazawa, A., Kono, N., Aoki, J.
ID
ZDB-PUB-220506-13
Date
2022
Source
Scientific Reports   12: 7312 (Journal)
Registered Authors
Nishina, Hiroshi
Keywords
none
MeSH Terms
  • Embryonic Development/genetics
  • 1-Acylglycerophosphocholine O-Acyltransferase/genetics
  • 1-Acylglycerophosphocholine O-Acyltransferase/metabolism
  • Down-Regulation
  • Reproduction/genetics
  • Animals
  • Fatty Acids*/metabolism
  • Female
  • Acyltransferases/metabolism
  • Zebrafish*/genetics
  • Zebrafish*/metabolism
  • Male
(all 12)
PubMed
35508627 Full text @ Sci. Rep.
Abstract
Phospholipids in the membrane consist of diverse pairs of fatty acids bound to a glycerol backbone. The biological significance of the diversity, however, remains mostly unclear. Part of this diversity is due to lysophospholipid acyltransferases (LPLATs), which introduce a fatty acid into lysophospholipids. The human genome has 14 LPLATs and most of them are highly conserved in vertebrates. Here, we analyzed the function of one of these enzymes, lysophosphatidylglycerol acyltransferase 1 (Lpgat1), in zebrafish. We found that the reproduction of heterozygous (lpgat1+/-) male mutants was abnormal. Crosses between heterozygous males and wild-type females produced many eggs with no obvious cleavage, whereas eggs produced by crosses between heterozygous females and wild-type males cleaved normally. Consistent with this, spermatozoa from heterozygous males had reduced motility and abnormal morphology. We also found that the occurrence of lpgat1 homozygous (lpgat1-/-) mutants was far lower than expected. In addition, downregulation of lpgat1 by morpholino antisense oligonucleotides resulted in severe developmental defects. Lipidomic analysis revealed that selective phospholipid species with stearic acid and docosahexaenoic acid were reduced in homozygous larvae and spermatozoa from heterozygotes. These results suggest that the specific phospholipid molecular species produced by Lpgat1 have an essential role in sperm fertilization and in embryonic development.
Genes / Markers
Figures
Figure Gallery (7 images)
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Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
lpgat1ABstandard conditionsAdultISH
1 - 1 of 1
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Phenotype
Fish Conditions Stage Phenotype Figure
AB + MO1-lpgat1 + MO2-lpgat1controlLong-pec
AB + MO1-lpgat1 + MO2-lpgat1controlLong-pec
AB + MO1-lpgat1 + MO2-lpgat1controlLong-pec
AB + MO1-lpgat1 + MO2-lpgat1controlProtruding-mouth
AB + MO1-lpgat1 + MO2-lpgat1controlProtruding-mouth
AB + MO1-lpgat1 + MO2-lpgat1controlProtruding-mouth
lpgat1zf3665/+ (AB)standard conditionsDays 7-13
lpgat1zf3665/+ (AB)standard conditionsDays 7-13
lpgat1zf3665/+ (AB)standard conditionsDays 7-13
lpgat1zf3665/+ (AB)standard conditionsDays 7-13
1 - 10 of 396
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
zf3665
    		Indel
    zf3666
      		Indel
      1 - 2 of 2
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      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      Target Reagent Reagent Type
      lpgat1CRISPR1-lpgat1CRISPR
      lpgat1CRISPR2-lpgat1CRISPR
      lpgat1MO1-lpgat1MRPHLNO
      lpgat1MO2-lpgat1MRPHLNO
      1 - 4 of 4
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      Fish
      Fish
      AB
      AB + MO1-lpgat1 + MO2-lpgat1
      lpgat1zf3665/+ (AB)
      lpgat1zf3665/zf3665 (AB)
      lpgat1zf3666/+ (AB)
      lpgat1zf3666/zf3666 (AB)
      1 - 6 of 6
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      Antibodies
      No data available
      Orthology
      No data available
      Engineered Foreign Genes
      No data available
      Mapping
      No data available
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      Citation
      Shibata, T., Kawana, H., Nishino, Y., Ito, Y., Sato, H., Onishi, H., Kano, K., Inoue, A., Taketomi, Y., Murakami, M., Kofuji, S., Nishina, H., Miyazawa, A., Kono, N., Aoki, J. (2022) Abnormal male reproduction and embryonic development induced by downregulation of a phospholipid fatty acid-introducing enzyme Lpgat1 in zebrafish. Scientific Reports. 12:7312.