PUBLICATION
Zebrafish obesogenic test identifies anti-adipogenic fraction in Moringa oreifera leaf extracts
- Authors
- Matsuoka, I., Hata, K., Katsuzaki, H., Nakayama, H., Zang, L., Ota, M., Kim, Y., Chu, D.C., Juneja, L.R., Nishimura, N., Shimada, Y.
- ID
- ZDB-PUB-220419-22
- Date
- 2022
- Source
- Food science & nutrition 10: 1248-1256 (Journal)
- Registered Authors
- Keywords
- diabetes, dyslipidemia, herbal medicine, natural products, visceral obesity
- MeSH Terms
- none
- PubMed
- 35432980 Full text @ Food Sci Nutr
Citation
Matsuoka, I., Hata, K., Katsuzaki, H., Nakayama, H., Zang, L., Ota, M., Kim, Y., Chu, D.C., Juneja, L.R., Nishimura, N., Shimada, Y. (2022) Zebrafish obesogenic test identifies anti-adipogenic fraction in Moringa oreifera leaf extracts. Food science & nutrition. 10:1248-1256.
Abstract
The zebrafish obesogenic test (ZOT) is a powerful tool for identifying anti-adipogenic compounds for in vivo screening. In our previous study, we found that Moringa oleifera (MO) leaf powder suppressed the accumulation of visceral adipose tissue (VAT) in ZOT. MO demonstrates a wide range of pharmacological effects; however, little is known about its functional constituents. To identify the anti-adipogenic components of MO leaves, we prepared extracts using different extraction methods and tested the obtained extracts and fractions using ZOT. We found that the dichloromethane extract and its hexane:EtOAc = 8:2 fraction reduced VAT accumulation in young zebrafish fed a high-fat diet. We also performed gene expression analysis in the zebrafish VAT and found that CCAAT/enhancer-binding protein beta and CCAAT/enhancer-binding protein delta (associated with early stages of adipogenesis) gene expression was downregulated after fraction 2 administration. We identified a new MO fraction that suppressed VAT accumulation by inhibiting early adipogenesis using the ZOT. Phenotype-driven zebrafish screening is a reasonable strategy for identifying bioactive components in natural products.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping