PUBLICATION
Optogenetic activators of apoptosis, necroptosis, and pyroptosis
- Authors
- Shkarina, K., Hasel de Carvalho, E., Santos, J.C., Ramos, S., Leptin, M., Broz, P.
- ID
- ZDB-PUB-220415-6
- Date
- 2022
- Source
- The Journal of cell biology 221(6): (Journal)
- Registered Authors
- Leptin, Maria
- Keywords
- none
- MeSH Terms
-
- Animals
- Apoptosis*/genetics
- Arabidopsis/genetics
- Cryptochromes/genetics
- Humans
- Lysophospholipids/metabolism
- Mice
- Necroptosis*/genetics
- Optogenetics*
- Pyroptosis*/genetics
- Sphingosine/analogs & derivatives
- Sphingosine/metabolism
- Zebrafish/genetics
- PubMed
- 35420640 Full text @ J. Cell Biol.
Citation
Shkarina, K., Hasel de Carvalho, E., Santos, J.C., Ramos, S., Leptin, M., Broz, P. (2022) Optogenetic activators of apoptosis, necroptosis, and pyroptosis. The Journal of cell biology. 221(6):.
Abstract
Targeted and specific induction of cell death in an individual or groups of cells hold the potential for new insights into the response of tissues or organisms to different forms of death. Here, we report the development of optogenetically controlled cell death effectors (optoCDEs), a novel class of optogenetic tools that enables light-mediated induction of three types of programmed cell death (PCD)-apoptosis, pyroptosis, and necroptosis-using Arabidopsis thaliana photosensitive protein Cryptochrome-2. OptoCDEs enable a rapid and highly specific induction of PCD in human, mouse, and zebrafish cells and are suitable for a wide range of applications, such as sub-lethal cell death induction or precise elimination of single cells or cell populations in vitro and in vivo. As the proof-of-concept, we utilize optoCDEs to assess the differences in neighboring cell responses to apoptotic or necrotic PCD, revealing a new role for shingosine-1-phosphate signaling in regulating the efferocytosis of the apoptotic cell by epithelia.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping