PUBLICATION
Apelin signaling dependent endocardial protrusions promote cardiac trabeculation in zebrafish
- Authors
- Qi, J., Rittershaus, A., Priya, R., Mansingh, S., Stainier, D.Y.R., Helker, C.S.M.
- ID
- ZDB-PUB-220302-19
- Date
- 2022
- Source
- eLIFE 11: (Journal)
- Registered Authors
- Helker, Christian, Priya, Rashmi, Rittershaus, Annegret, Stainier, Didier
- Keywords
- cell biology, developmental biology, zebrafish
- MeSH Terms
-
- Animals
- Apelin/metabolism
- Endocardium*/metabolism
- Myocytes, Cardiac/metabolism
- Neuregulins/metabolism
- Zebrafish*/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 35225788 Full text @ Elife
Citation
Qi, J., Rittershaus, A., Priya, R., Mansingh, S., Stainier, D.Y.R., Helker, C.S.M. (2022) Apelin signaling dependent endocardial protrusions promote cardiac trabeculation in zebrafish. eLIFE. 11:.
Abstract
During cardiac development, endocardial cells (EdCs) produce growth factors to promote myocardial morphogenesis and growth. In particular, EdCs produce Neuregulin which is required for ventricular cardiomyocytes (CMs) to seed the multicellular ridges known as trabeculae. Defects in Neuregulin signaling, or in endocardial sprouting towards CMs, cause hypotrabeculation. However, the mechanisms underlying endocardial sprouting remain largely unknown. Here, we first show by live imaging in zebrafish embryos that EdCs interact with CMs via dynamic membrane protrusions. After touching CMs, these protrusions remain in close contact with their target despite the vigorous cardiac contractions. Loss of the CM-derived peptide Apelin, or of the Apelin receptor, which is expressed in EdCs, leads to reduced endocardial sprouting and hypotrabeculation. Mechanistically, Neuregulin signaling requires endocardial protrusions to induce extracellular signal-regulated kinase (Erk) activity in CMs and trigger their delamination. Altogether, these data show that Apelin signaling dependent endocardial protrusions modulate CM behavior during trabeculation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping