PUBLICATION
Stab Wound Injury Model of the Adult Optic Tectum using Zebrafish and Medaka for the Comparative Analysis of Regenerative Capacity
- Authors
- Shimizu, Y., Kawasaki, T.
- ID
- ZDB-PUB-220302-17
- Date
- 2022
- Source
- Journal of visualized experiments : JoVE (180): (Journal)
- Registered Authors
- Shimizu, Yuki
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Proliferation/physiology
- Neurogenesis/physiology
- Oryzias*
- Superior Colliculi
- Wounds, Stab*/pathology
- Zebrafish
- PubMed
- 35225278 Full text @ J. Vis. Exp.
Citation
Shimizu, Y., Kawasaki, T. (2022) Stab Wound Injury Model of the Adult Optic Tectum using Zebrafish and Medaka for the Comparative Analysis of Regenerative Capacity. Journal of visualized experiments : JoVE. (180):.
Abstract
While zebrafish have a superior capacity to regenerate their central nervous system (CNS), medaka has a lower CNS regenerative capacity. A brain injury model was developed in the adult optic tectum of zebrafish and medaka and comparative histological and molecular analyses were performed to elucidate the molecular mechanisms regulating the high regenerative capacity of this tissue across these fish species. Here a stab wound injury model is presented for the adult optic tectum using a needle and histological analyses for proliferation and differentiation of the neural stem cells (NSCs). A needle was manually inserted into the central region of the optic tectum, and then the fish were intracardially perfused, and their brains were dissected. These tissues were then cryosectioned and evaluated using immunostaining against the appropriate NSC proliferation and differentiation markers. This tectum injury model provides robust and reproducible results in both zebrafish and medaka, allowing for comparing NSC responses after injury. This method is available for small teleosts, including zebrafish, medaka, and African killifish, and enables us to compare their regenerative capacity and investigate unique molecular mechanisms.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping