PUBLICATION
MPTP-Treated Zebrafish Recapitulate 'Late-Stage' Parkinson's-like Cognitive Decline
- Authors
- Bashirzade, A.A.O., Cheresiz, S.V., Belova, A.S., Drobkov, A.V., Korotaeva, A.D., Azizi-Arani, S., Azimirad, A., Odle, E., Gild, E.V., Ardashov, O.V., Volcho, K.P., Bozhko, D.V., Myrov, V.O., Kolchanova, S.M., Polovian, A.I., Galumov, G.K., Salakhutdinov, N.F., Amstislavskaya, T.G., Kalueff, A.V.
- ID
- ZDB-PUB-220225-12
- Date
- 2022
- Source
- Toxics 10(2): (Journal)
- Registered Authors
- Keywords
- 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), Parkinson’s disease, artificial intelligence, inhibitory avoidance task, spontaneous alternation, zebrafish
- MeSH Terms
- none
- PubMed
- 35202255 Full text @ Toxics
Citation
Bashirzade, A.A.O., Cheresiz, S.V., Belova, A.S., Drobkov, A.V., Korotaeva, A.D., Azizi-Arani, S., Azimirad, A., Odle, E., Gild, E.V., Ardashov, O.V., Volcho, K.P., Bozhko, D.V., Myrov, V.O., Kolchanova, S.M., Polovian, A.I., Galumov, G.K., Salakhutdinov, N.F., Amstislavskaya, T.G., Kalueff, A.V. (2022) MPTP-Treated Zebrafish Recapitulate 'Late-Stage' Parkinson's-like Cognitive Decline. Toxics. 10(2):.
Abstract
The zebrafish is a promising model species in biomedical research, including neurotoxicology and neuroactive drug screening. 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) evokes degeneration of dopaminergic neurons and is commonly used to model Parkinson's disease (PD) in laboratory animals, including zebrafish. However, cognitive phenotypes in MPTP-evoked experimental PD models remain poorly understood. Here, we established an LD50 (292 mg/kg) for intraperitoneal MPTP administration in adult zebrafish, and report impaired spatial working memory (poorer spontaneous alternation in the Y-maze) in a PD model utilizing fish treated with 200 µg of this agent. In addition to conventional behavioral analyses, we also employed artificial intelligence (AI)-based approaches to independently and without bias characterize MPTP effects on zebrafish behavior during the Y-maze test. These analyses yielded a distinct cluster for 200-μg MPTP (vs. other) groups, suggesting that high-dose MPTP produced distinct, computationally detectable patterns of zebrafish swimming. Collectively, these findings support MPTP treatment in adult zebrafish as a late-stage experimental PD model with overt cognitive phenotypes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping