PUBLICATION
Enhancement of Edwardsiella piscicida infection, biofilm formation, and motility caused by N-acetylneuraminate lyase
- Authors
- Vo, L.K., Tran, N.T., Kubo, Y., Sahashi, D., Komatsu, M., Shiozaki, K.
- ID
- ZDB-PUB-220223-6
- Date
- 2022
- Source
- Glycoconjugate journal 39(3): 429-442 (Journal)
- Registered Authors
- Shiozaki, Kazuhiro
- Keywords
- Dihydrodipicolinate synthase, Edwardsiella piscicida, N-acetylneuraminate lyase, Sialic acid catabolism
- MeSH Terms
-
- Animals
- Bacterial Proteins/genetics
- Bacterial Proteins/metabolism
- Biofilms
- Edwardsiella
- N-Acetylneuraminic Acid*/metabolism
- Oxo-Acid-Lyases
- Zebrafish*
- PubMed
- 35192095 Full text @ Glycoconj. J.
Citation
Vo, L.K., Tran, N.T., Kubo, Y., Sahashi, D., Komatsu, M., Shiozaki, K. (2022) Enhancement of Edwardsiella piscicida infection, biofilm formation, and motility caused by N-acetylneuraminate lyase. Glycoconjugate journal. 39(3):429-442.
Abstract
Sialic acid and its catabolism are involved in bacterial pathogenicity. N-acetylneuraminate lyase (NAL), which catalyzes the reversible aldol cleavage of sialic acid to form N-acetyl-D-mannosamine in the first step of sialic acid degradation, has been recently investigated to elucidate whether NAL enhances bacterial virulence; however, the role of NAL in bacterial pathogenicity remains unclear. In the present study, we demonstrated that the existence of two enzymes in Edwardsiella piscicida, referred to as dihydrodipicolinate synthase (DHDPS) and NAL, induced the cleavage/condensation activity toward sialic acids such as N-acetylneuraminic acid, N-glycolylneuraminic acid and 3-deoxy-D-glycero-D-galacto-non-2-ulopyranosonic acid. NAL enhanced cellular infection in vitro and suppressed the survival rate in zebrafish larvae in bath-infection in vivo, whereas DHDPS did not. Furthermore, NAL strongly activated the expression of E. piscicida phenotypes such as biofilm formation and motility, whereas DHDPS did not. Besides, the gene expression level of nanK, nanE, and glmU were up-regulated in the NAL-overexpressing strain, along with an increase in the total amount of N-acetylglucosamine.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping