PUBLICATION

Osteoblasts pattern endothelium and somatosensory axons during zebrafish caudal fin organogenesis

Authors
Bump, R.G., Goo, C.E.A., Horton, E.C., Rasmussen, J.P.
ID
ZDB-PUB-220208-19
Date
2022
Source
Development (Cambridge, England)   149(3): (Journal)
Registered Authors
Rasmussen, Jeff
Keywords
Danio rerio, Bone, Developmental biology, Neurovascular congruence, Somatosensory neuron, VEGFR
MeSH Terms
  • Animals, Genetically Modified/growth & development
  • Animals, Genetically Modified/metabolism
  • Organogenesis/physiology*
  • Intracellular Signaling Peptides and Proteins/genetics
  • Intracellular Signaling Peptides and Proteins/metabolism
  • Endothelium/cytology
  • Endothelium/metabolism*
  • Larva/growth & development
  • Larva/metabolism
  • Zebrafish/growth & development*
  • Zebrafish/metabolism
  • Axons/metabolism*
  • Receptors, Vascular Endothelial Growth Factor/metabolism
  • Osteoblasts/cytology
  • Osteoblasts/metabolism
  • Sp7 Transcription Factor/metabolism
  • Animals
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Animal Fins/growth & development
  • Animal Fins/physiology*
(all 21)
PubMed
35129199 Full text @ Development
Abstract
Skeletal elements frequently associate with vasculature and somatosensory nerves, which regulate bone development and homeostasis. However, the deep, internal location of bones in many vertebrates has limited in vivo exploration of the neurovascular-bone relationship. Here, we use the zebrafish caudal fin, an optically accessible organ formed of repeating bony ray skeletal units, to determine the cellular relationship between nerves, bones and endothelium. In adult zebrafish, we establish the presence of somatosensory axons running through the inside of the bony fin rays, juxtaposed with osteoblasts on the inner hemiray surface. During development we show that the caudal fin progresses through sequential stages of endothelial plexus formation, bony ray addition, ray innervation and endothelial remodeling. Surprisingly, the initial stages of fin morphogenesis proceed normally in animals lacking either fin endothelium or somatosensory nerves. Instead, we find that sp7+ osteoblasts are required for endothelial remodeling and somatosensory axon innervation in the developing fin. Overall, this study demonstrates that the proximal neurovascular-bone relationship in the adult caudal fin is established during fin organogenesis and suggests that ray-associated osteoblasts pattern axons and endothelium.
Genes / Markers
Figures
Figure Gallery (7 images)
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
a129TgTransgenic Insertion
    b1212TgTransgenic Insertion
      la207TgTransgenic Insertion
        pd46TgTransgenic Insertion
          s984
            		Indel
            sd27TgTransgenic Insertion
              st48
                		Point Mutation
                w61TgTransgenic Insertion
                  y1TgTransgenic Insertion
                    zf384TgTransgenic Insertion
                      1 - 10 of 10
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                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      No data available
                      Fish
                      No data available
                      Antibodies
                      Name Type Antigen Genes Isotypes Host Organism
                      Ab15-tubamonoclonal
                        		IgGRabbit
                        zn-12monoclonal
                          		IgG1Mouse
                          zns-5monoclonal
                            		IgG1Mouse
                            1 - 3 of 3
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                            Orthology
                            No data available
                            Engineered Foreign Genes
                            Marker Marker Type Name
                            BFPEFGBFP
                            CreEFGCre
                            DsRedEFGDsRed
                            EGFPEFGEGFP
                            mCherryEFGmCherry
                            1 - 5 of 5
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                            Mapping
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                            Citation
                            Bump, R.G., Goo, C.E.A., Horton, E.C., Rasmussen, J.P. (2022) Osteoblasts pattern endothelium and somatosensory axons during zebrafish caudal fin organogenesis. Development (Cambridge, England). 149(3):.