PUBLICATION
The neuroprotective effects of Galectin-1 on Parkinson's Disease via regulation of Nrf2 expression
- Authors
- Liu, H.B., Li, Q.Y., Zhang, X.D., Shi, Y., Li, J.Y.
- ID
- ZDB-PUB-220204-5
- Date
- 2022
- Source
- European review for medical and pharmacological sciences 26: 623-636 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- 1-Methyl-4-phenylpyridinium/toxicity
- Animals
- Apoptosis
- Cell Line, Tumor
- Cell Survival
- Galectin 1/genetics
- Galectin 1/metabolism
- Galectin 1/pharmacology
- NF-E2-Related Factor 2/metabolism
- Neuroprotective Agents*/pharmacology
- Oxidative Stress
- Parkinson Disease*/drug therapy
- Parkinson Disease*/genetics
- Reactive Oxygen Species/metabolism
- Zebrafish/metabolism
- PubMed
- 35113438 Full text @ Eur Rev Med Pharmacol Sci
Citation
Liu, H.B., Li, Q.Y., Zhang, X.D., Shi, Y., Li, J.Y. (2022) The neuroprotective effects of Galectin-1 on Parkinson's Disease via regulation of Nrf2 expression. European review for medical and pharmacological sciences. 26:623-636.
Abstract
Objective Parkinson's disease (PD) is one of the neurodegenerative diseases. Galectin-1 (Gal-1) is expressed in the central nervous system. Our study sought to explore the neuroprotective effect of Gal-1 in 1‑methyl‑4‑phenyl pyridine ion (MPP+)-induced cytotoxicity on SH-SY5Y cells.
Materials and methods SH-SY5Y cells were cultured in vitro, pretreated with Gal-1, and then exposed to MPP+. Thereafter, the generation of reactive oxygen species (ROS) in SH-SY5Y cells was investigated. The effects of Gal-1 on DNA breakage, cell damage (release of lactate dehydrogenase (LDH)), viability, and apoptosis in SH-SY5Y cells were examined by comet assay, LDH assay, WST-1 assay, and flow cytometry, respectively. Additionally, the regulatory effect of Gal-1 on Nrf2 expression was examined by western blot. Zebrafish embryos were pretreated with Gal-1 and then exposed to MPP+. The locomotor ability of zebrafish larvae was then investigated.
Results MPP+ induced the production of ROS in cells, which can be alleviated by pretreatment with Gal-1. Gal-1 protected cells from MPP+-induced cytotoxicity by preventing DNA breakage and cell injury. Gal-1 inhibited apoptosis in SH-SY5Y cells. The neuroprotective effect of Gal-1 could be abolished when Nrf2 expression knockdown. Moreover, exposure to MPP+ decreased the locomotor activity of zebrafish, which was attenuated by pretreatment with Gal-1.
Conclusions Our study demonstrated that the administration of Gal-1 could protect neurons from cellular stress by preventing apoptosis and eliminating ROS. Moreover, the neuroprotective effect of Gal-1 in neuronal cells could be related to the activation of Nrf2 expression. Therefore, Gal-1 could be a promising strategy for treating PD.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping