PUBLICATION
Distribution of Neurogranin-like immunoreactivity in the brain and sensory organs of the adult Zebrafish
- Authors
- Alba-González, A., Folgueira, M., Castro, A., Anadón, R., Yáñez, J.
- ID
- ZDB-PUB-220112-12
- Date
- 2022
- Source
- The Journal of comparative neurology 530(10): 1569-1587 (Journal)
- Registered Authors
- Folgueira Otero, Monica
- Keywords
- Danio rerio, RC3, neurogranin, protein kinase C, teleost, zebrafish
- MeSH Terms
-
- Animals
- Brain/metabolism
- Diencephalon/metabolism
- Mammals
- Neurogranin*/analysis
- Neurogranin*/metabolism
- Rhombencephalon/chemistry
- Zebrafish*/metabolism
- PubMed
- 35015905 Full text @ J. Comp. Neurol.
Citation
Alba-González, A., Folgueira, M., Castro, A., Anadón, R., Yáñez, J. (2022) Distribution of Neurogranin-like immunoreactivity in the brain and sensory organs of the adult Zebrafish. The Journal of comparative neurology. 530(10):1569-1587.
Abstract
We studied the expression of neurogranin in the brain and some sensory organs (barbel taste buds, olfactory organ and retina) of adult zebrafish. Database analysis shows zebrafish has two paralog neurogranin genes (nrgna and nrgnb) that translate into three peptides with a conserved IQ-domain, as in mammals. Western blots of zebrafish brain extracts using an anti-neurogranin antiserum revealed three separate bands, confirming the presence of three neurogranin peptides. Immunohistochemistry shows neurogranin-like expression in the brain and sensory organs (taste buds, neuromasts and olfactory epithelium), not being able to discern its three different peptides. In the retina, the most conspicuous positive cells were bipolar neurons. In the brain, immunopositive neurons were observed in all major regions (pallium, subpallium, preoptic area, hypothalamus, diencephalon, mesencephalon and rhombencephalon, including the cerebellum), a more extended distribution than in mammals. Interestingly, dendrites, cell bodies and axon terminals of some neurons were immunopositive, thus zebrafish neurogranins may play presynaptic and postsynaptic roles. Most positive neurons were found in primary sensory centers (viscerosensory column and medial octavolateral nucleus) and integrative centers (pallium, subpallium, optic tectum and cerebellum), which have complex synaptic circuitry. However, we also observed expression in areas not related to sensory or integrative functions, such as in CSF-contacting cells associated with the hypothalamic recesses, which exhibited high neurogranin-like immunoreactivity. Together, these results reveal important differences with the patterns reported in mammals, suggesting divergent evolution from the common ancestor. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping