PUBLICATION
5-Hydroxymethyl-2-furaldehyde induces developmental toxicology and decreases bone mineralization in zebrafish larvae
- Authors
- Jiang, Y., Zhong, Z., Wang, M., Zhang, X.
- ID
- ZDB-PUB-220104-5
- Date
- 2021
- Source
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 254: 109254 (Journal)
- Registered Authors
- Zhong, Zhaomin
- Keywords
- 5-Hydroxymethyl-2-furaldehyde, Bone mineralization, Developmental toxicology, Reactive oxygen species, Zebrafish
- MeSH Terms
-
- Animals
- Calcification, Physiologic
- Embryo, Nonmammalian*
- Furaldehyde/analogs & derivatives
- Larva
- Zebrafish*/metabolism
- PubMed
- 34971842 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
Citation
Jiang, Y., Zhong, Z., Wang, M., Zhang, X. (2021) 5-Hydroxymethyl-2-furaldehyde induces developmental toxicology and decreases bone mineralization in zebrafish larvae. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 254:109254.
Abstract
In this study, we aimed to assess the developmental toxicity and effects of 5-HMF in zebrafish as a model organism for toxicology studies. To this end, we treated zebrafish embryos with 1-100 μg/mL 5-HMF and observed bone staining, gene expression, and reactive oxygen species levels in order to investigate the toxicological effects of 5-HMF. The results showed that high concentrations of 5-HMF caused increased mortality and deformity rates in zebrafish larvae, inhibited cartilage development, reduced bone mineralization, increased reactive oxygen species levels, and disrupted the expression of genes related to bone development and reactive oxygen species enzyme activity. The antioxidant N-acetyl-l-cysteine partially rescued the toxicological effects caused by the high concentrations of 5-HMF. Overall, these findings showed that high concentrations of 5-HMF induce reactive oxygen species production, leading to developmental toxicity and decreased bone mineralization. Our results provide a reference for understanding the toxic effects of 5-HMF.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping