PUBLICATION
Preparation and structural properties of selenium modified heteropolysaccharide from the fruits of Akebia quinata and in vitro and in vivo antitumor activity
- Authors
- Wang, H., Li, Y., Wang, X., Li, Y., Cui, J., Jin, D.Q., Tuerhong, M., Abudukeremu, M., Xu, J., Guo, Y.
- ID
- ZDB-PUB-220104-10
- Date
- 2022
- Source
- Carbohydrate Polymers 278: 118950 (Journal)
- Registered Authors
- Cui, Jianlin
- Keywords
- Akebia quinata, Antitumor activity, Dichloromethane (PubChem CID: 6344), Ethanol (PubChem CID: 702), Heteropolysaccharide, Methyl iodide (PubChem CID: 6328), Neutral red (PubChem CID: 11105), O-tolylisothiocyanate (PubChem CID: 341280687), Selenylation, Sodium chloride (PubChem CID: 5234), Sodium dihydrogen phosphate (PubChem CID: 23672064), Structure characterization, d-Glucose (PubChem CID: 5793), d-Mannose (PubChem CID: 18950), l-Arabinose (PubChem CID: 439195)
- MeSH Terms
-
- Angiogenesis Inhibitors/chemistry
- Angiogenesis Inhibitors/isolation & purification
- Angiogenesis Inhibitors/pharmacology*
- Animals
- Antineoplastic Agents, Phytogenic/chemistry
- Antineoplastic Agents, Phytogenic/isolation & purification
- Antineoplastic Agents, Phytogenic/pharmacology*
- Carbohydrate Conformation
- Cell Movement/drug effects
- Cell Proliferation/drug effects
- Drug Screening Assays, Antitumor
- Fruit/chemistry*
- Hep G2 Cells
- Humans
- Liver Neoplasms, Experimental/drug therapy
- Liver Neoplasms, Experimental/pathology
- Neovascularization, Pathologic/drug therapy*
- Neovascularization, Pathologic/pathology
- Polysaccharides/chemistry
- Polysaccharides/isolation & purification
- Polysaccharides/pharmacology*
- Ranunculales/chemistry*
- Selenium/chemistry*
- Zebrafish/embryology
- PubMed
- 34973766 Full text @ Carbohydr. Polym.
Citation
Wang, H., Li, Y., Wang, X., Li, Y., Cui, J., Jin, D.Q., Tuerhong, M., Abudukeremu, M., Xu, J., Guo, Y. (2022) Preparation and structural properties of selenium modified heteropolysaccharide from the fruits of Akebia quinata and in vitro and in vivo antitumor activity. Carbohydrate Polymers. 278:118950.
Abstract
Cancer is a complex disease, and blocking tumor angiogenesis has become one of the most promising approaches in cancer therapy. Here, an exopoly heteropolysaccharide (AQP70-2B) was firstly isolated from Akebia quinata. Monosaccharide composition indicated that the AQP70-2B was composed of rhamnose, glucose, galactose, and arabinose. The backbone of AQP70-2B consisted of →1)-l-Araf, →3)-l-Araf-(1→, →5)-l-Araf-(1→, →3,5)-l-Araf-(1→, →2,5)-l-Araf-(1→, →4)-d-Glcp-(1→, →6)-d-Galp-(1→, and →1)-d-Rhap residues. Based on the close relationship between selenium and anti-tumor activity, AQP70-2B was modified with selenium to obtain selenized polysaccharide Se-AQP70-2B. Then, a series of methods for analysis and characterization, especially scanning electron microscopy coupled with energy dispersive spectrometry (SEM-EDS), indicated that Se-AQP70-2B was successfully synthesized. Furthermore, zebrafish xenografts and anti-angiogenesis experiments indicated that selenization could improve the antitumor activity by inhibiting tumor cell proliferation and migration and blocking angiogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping