PUBLICATION
Rapid Mounting of Zebrafish Larvae for Brain Imaging
- Authors
- Geng, Y., Peterson, R.T.
- ID
- ZDB-PUB-211224-9
- Date
- 2021
- Source
- Zebrafish 18: 376-379 (Journal)
- Registered Authors
- Peterson, Randall
- Keywords
- brain imaging, computer numerical control, rapid mounting
- MeSH Terms
-
- Animals
- Brain*
- Larva
- Neuroimaging
- Zebrafish*
- PubMed
- 34935498 Full text @ Zebrafish
Citation
Geng, Y., Peterson, R.T. (2021) Rapid Mounting of Zebrafish Larvae for Brain Imaging. Zebrafish. 18:376-379.
Abstract
Brain imaging requires mounting of zebrafish larvae in a vertical position, but anesthetized or fixed larvae tend to fall on their sides without external support. Current solution is to manually hold individual larva until liquid agarose solidifies, which is time consuming, labor intensive, and unfriendly to beginners. We developed a method to form larva-shaped slots in agarose gel using a computer numerical controlled manufactured mold. Each slot nearly perfectly fits a larva in its upright position, and larvae can be easily mounted by inserting into the slots. On average, each larva can be mounted in <1 min using this method.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping