PUBLICATION
Wholemount In-Situ Hybridization (WISH) in Zebrafish Embryos to Analyze Craniofacial Development
- Authors
- Mathiyalagan, N., Dworkin, S.
- ID
- ZDB-PUB-211217-6
- Date
- 2022
- Source
- Methods in molecular biology (Clifton, N.J.) 2403: 19-32 (Other)
- Registered Authors
- Dworkin, Seb
- Keywords
- Craniofacial, Gene expression, In-situ hybridization, Neural crest, Riboprobe, Zebrafish
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian/metabolism
- Gene Expression Regulation, Developmental
- In Situ Hybridization
- Neural Crest/metabolism
- Skull
- Zebrafish*/genetics
- Zebrafish*/metabolism
- Zebrafish Proteins/metabolism
- PubMed
- 34913113 Full text @ Meth. Mol. Biol.
Citation
Mathiyalagan, N., Dworkin, S. (2022) Wholemount In-Situ Hybridization (WISH) in Zebrafish Embryos to Analyze Craniofacial Development. Methods in molecular biology (Clifton, N.J.). 2403:19-32.
Abstract
Wholemount in-situ hybridization in zebrafish is a powerful technique for visualizing spatiotemporal gene expression during development. Here we describe a technique to detect endogenous mRNA expression in zebrafish that can be adapted to use on embryos from the single-cell stage until 5 days postfertilization.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping