PUBLICATION
Systematic expression profiling of neuropathy-related aminoacyl-tRNA synthetases in zebrafish during development
- Authors
- Giong, H.K., Lee, J.S.
- ID
- ZDB-PUB-211207-27
- Date
- 2021
- Source
- Biochemical and Biophysical Research Communications 587: 92-98 (Journal)
- Registered Authors
- Giong, Hoi-Khoanh, Lee, Jeong-Soo
- Keywords
- Aminoacyl tRNA synthetases, Expression, In situ, Neuropathy, Systematic
- MeSH Terms
-
- Molecular Sequence Annotation
- Animals
- Humans
- Muscles/metabolism
- Zebrafish/genetics*
- PubMed
- 34872004 Full text @ Biochem. Biophys. Res. Commun.
Abstract
Aminoacyl tRNA synthetases (ARSs) are a group of proteins, acting as transporters to transfer and attach the appropriate amino acids onto their cognate tRNAs for translation. So far, 18 out of 20 cytoplasmic ARSs are reported to be connected to different neuropathy disorders with multi-organ defects that are often accompanied with developmental delays. Thus, it is important to understand functions and impacts of ARSs at the whole organism level. Here, we systematically analyzed the spatiotemporal expression of 14 ars and 2 aimp genes during development in zebrafish that have not be previously reported. Not only in the brain, their dynamic expression patterns in several tissues such as in the muscles, liver and intestine suggest diverse roles in a wide range of development processes in addition to neuronal function, which is consistent with potential involvement in multiple syndrome diseases associated with ARS mutations. In particular, hinted by its robust expression pattern in the brain, we confirmed that aimp1 is required for the formation of cerebrovasculature by a loss-of-function approach. Overall, our systematic profiling data provides a useful basis for studying roles of ARSs during development and understanding their potential functions in the etiology of related diseases.
Genes / Markers
Figure Gallery (4 images)
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping