PUBLICATION
Tissue distribution and endocrine disruption effects of chronic exposure to pharmaceuticals and personal care products mixture at environmentally relevant concentrations in zebrafish
- Authors
- Hamid, N., Junaid, M., Manzoor, R., Duan, J.J., Lv, M., Xu, N., Pei, D.S.
- ID
- ZDB-PUB-211203-6
- Date
- 2021
- Source
- Aquatic toxicology (Amsterdam, Netherlands) 242: 106040 (Journal)
- Registered Authors
- Junaid, Muhammad, Pei, Desheng
- Keywords
- Estrogen receptors, HPG axis, Molecular docking, PPCPs toxicity, Xenoestrogens
- MeSH Terms
-
- Animals
- Cosmetics*/toxicity
- Environmental Monitoring
- Female
- Male
- Molecular Docking Simulation
- Pharmaceutical Preparations*
- Tandem Mass Spectrometry
- Tissue Distribution
- Water Pollutants, Chemical*/toxicity
- Zebrafish
- PubMed
- 34856459 Full text @ Aquat. Toxicol.
Citation
Hamid, N., Junaid, M., Manzoor, R., Duan, J.J., Lv, M., Xu, N., Pei, D.S. (2021) Tissue distribution and endocrine disruption effects of chronic exposure to pharmaceuticals and personal care products mixture at environmentally relevant concentrations in zebrafish. Aquatic toxicology (Amsterdam, Netherlands). 242:106040.
Abstract
Pharmaceuticals and personal care products (PPCPs) as emerging contaminants are ubiquitously present in the aquatic environment. Using in vivo and in silico techniques, this study aims to elucidate tissue distribution and endocrine disruption effects of chronic exposure (120 days) to PPCP mixture at environmentally relevant concentrations (ERCs) in adult zebrafish. Results from UHPLC-MS/MS analyses showed elevated distribution of PPCPs in zebrafish tissues in the order of liver > gonad > brain. Upregulation of steroid hormone receptors, both gonadotropin, and steroidogenic genes perturb the HPG axis pathway in females, while male fish exhibited significantly downregulated expressions of vtg, cyp17, and 17βhsd genes with inhibited fecundity. The Spearman correlation indicated a significant positive relationship between PPCPs bioaccumulation and mRNA levels of HPG axis genes. In silico molecular docking (MD) revealed specific amino acid residues of PPCPs binding with zebrafish estrogen receptors. Furthermore, the strongest binding energies of sulfamethoxazole, carbamazepine, and triclosan were discovered in erα and erβ estrogen receptors, confirming PPCPs' xenoestrogenic behavior. To summarize, chronic exposure to ERCs resulted in a high accumulation of PPCPs in the liver and gonad tissues of adult zebrafish, as well as associated perturbed genetic responses. As a result, strict environmental regulations for the disposal of PPCPs should be ensured to protect ecological and public health.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping