PUBLICATION

Tetraspanin Cd9b and Cxcl12a/Cxcr4b have a synergistic effect on the control of collective cell migration

Authors
Marsay, K.S., Greaves, S., Mahabaleshwar, H., Ho, C.M., Roehl, H., Monk, P.N., Carney, T.J., Partridge, L.J.
ID
ZDB-PUB-211201-9
Date
2021
Source
PLoS One   16: e0260372 (Journal)
Registered Authors
Carney, Tom, Mahabaleshwar, Harsha, Roehl, Henry
Keywords
none
MeSH Terms
  • Chemokine CXCL12/genetics
  • Chemokine CXCL12/metabolism*
  • Receptors, CXCR4/genetics
  • Receptors, CXCR4/metabolism*
  • Tetraspanin 29/genetics
  • Tetraspanin 29/metabolism*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Cell Movement*
  • Signal Transduction*
  • Animals, Genetically Modified/embryology
  • Animals, Genetically Modified/genetics
  • Gene Knockdown Techniques
  • Animals
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
(all 16)
PubMed
34847198 Full text @ PLoS One
Abstract
Collective cell migration is essential for embryonic development and homeostatic processes. During zebrafish development, the posterior lateral line primordium (pLLP) navigates along the embryo flank by collective cell migration. The chemokine receptors, Cxcr4b and Cxcr7b, as well as their cognate ligand, Cxcl12a, are essential for this process. We corroborate that knockdown of the zebrafish cd9 tetraspanin orthologue, cd9b, results in mild pLL abnormalities. Through generation of CRISPR and TALEN mutants, we show that cd9a and cd9b function partially redundantly in pLLP migration, which is delayed in the cd9b single and cd9a; cd9b double mutants. This delay led to a transient reduction in neuromast numbers. Loss of both Cd9a and Cd9b sensitized embryos to reduced Cxcr4b and Cxcl12a levels. Together these results provide evidence that Cd9 modulates collective cell migration of the pLLP during zebrafish development. One interpretation of these observations is that Cd9 contributes to more effective chemokine signalling.
Genes / Markers
Figures
Figure Gallery (7 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
sh474
    		Indel
    sh695
      		Small Deletion
      zf106TgTransgenic Insertion
        zf3825
          		Indel
          1 - 4 of 4
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          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          Target Reagent Reagent Type
          cd9aCRISPR1-cd9aCRISPR
          cd9bMO1-cd9bMRPHLNO
          cd9bMO2-cd9bMRPHLNO
          cd9bTALEN1-cd9bTALEN
          cxcl12aMO4-cxcl12aMRPHLNO
          cxcr4bMO1-cxcr4bMRPHLNO
          1 - 6 of 6
          Show
          Fish
          No data available
          Antibodies
          No data available
          Orthology
          No data available
          Engineered Foreign Genes
          Marker Marker Type Name
          EGFPEFGEGFP
          1 - 1 of 1
          Show
          Mapping
          No data available
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          Citation
          Marsay, K.S., Greaves, S., Mahabaleshwar, H., Ho, C.M., Roehl, H., Monk, P.N., Carney, T.J., Partridge, L.J. (2021) Tetraspanin Cd9b and Cxcl12a/Cxcr4b have a synergistic effect on the control of collective cell migration. PLoS One. 16:e0260372.