PUBLICATION
A novel low-molecular-weight chitosan/gamma-polyglutamic acid polyplexes for nucleic acid delivery into zebrafish larvae
- Authors
- Leung, S.W., Cheng, P.C., Chou, C.M., Lin, C., Kuo, Y.C., Lee, Y.A., Liu, C.Y., Mi, F.L., Cheng, C.H.
- ID
- ZDB-PUB-211129-38
- Date
- 2021
- Source
- International journal of biological macromolecules 194: 384-394 (Journal)
- Registered Authors
- Cheng, Chia-Hsiung
- Keywords
- Delivery, Gamma-polyglutamic acid, Innate immunity, Low-molecular-weight chitosan, Polyplex, Zebrafish
- MeSH Terms
-
- Animals
- Cell Survival
- Cells, Cultured
- Chemical Phenomena
- Chitosan/chemistry*
- Drug Carriers/chemical synthesis
- Drug Carriers/chemistry*
- Gene Expression
- Gene Transfer Techniques*
- Genes, Reporter
- Larva
- Molecular Weight
- Nucleic Acids/administration & dosage*
- Polyglutamic Acid/analogs & derivatives*
- Polyglutamic Acid/chemical synthesis
- Polyglutamic Acid/chemistry
- Spectrum Analysis
- Zebrafish
- PubMed
- 34822829 Full text @ Int. J. Biol. Macromol.
Citation
Leung, S.W., Cheng, P.C., Chou, C.M., Lin, C., Kuo, Y.C., Lee, Y.A., Liu, C.Y., Mi, F.L., Cheng, C.H. (2021) A novel low-molecular-weight chitosan/gamma-polyglutamic acid polyplexes for nucleic acid delivery into zebrafish larvae. International journal of biological macromolecules. 194:384-394.
Abstract
Many challenges, such as virus infection, extreme weather and long cultivation periods, during the development of fish larvae have been observed, especially in aquaculture. Gene delivery is a useful method to express functional genes to defend against these challengers. However, the methods for fish larvae are insufficient. In our earlier report, low-molecular-weight chitosan (LMWCS) showed a strong positive charge and may be useful for polyplex formulation. Herein, we present a simple self-assembly of LMWCS polyplexes (LMWCSrNPs) for gene delivery into zebrafish larvae. Different weight ratios of LMWCS/gamma-polyglutamic acid (γ-PGA)/plasmid DNA were analyzed by gel mobility assay. Delivery efficiency determined by green fluorescent protein (GFP) expression in zebrafish liver (ZFL) cells showed that delivery efficiency at a weight ratio of 20:8:1 was higher than others. Zeta potential and transmission electron microscopy (TEM) analysis showed that the round shape of the particle size varied. In our earlier reports, IRF9S2C could induce interferon-stimulated gene (ISG) expression to induce innate immunity in zebrafish and pufferfish. Further delivery of pcDNA3-IRF9S2C-HA plasmid DNA into ZFL cells and zebrafish larvae by LMWCSrNP successfully induced ISG expression. Collectively, LMWCSrNP could be a novel gene delivery system for zebrafish larvae and might be used to improve applications in aquaculture.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping