PUBLICATION
In Vitro and In Vivo Toxicity Evaluation of Natural Products with Potential Applications as Biopesticides
- Authors
- Sangermano, F., Masi, M., Kumar, A., Peravali, R., Tuzi, A., Cimmino, A., Vallone, D., Giamundo, G., Conte, I., Evidente, A., Calabrò, V.
- ID
- ZDB-PUB-211129-34
- Date
- 2021
- Source
- toxins 13(11): (Journal)
- Registered Authors
- Peravali, Ravindra, Vallone, Daniela
- Keywords
- DNA damage, agricultural application, cyclopaldic and α-costic acids, cytotoxicity, developmental toxicity, fish embryos, plant and microbial metabolites
- MeSH Terms
-
- Animals
- Biological Control Agents/toxicity*
- Biological Products/toxicity*
- Cell Line
- Embryo, Nonmammalian/drug effects*
- Humans
- Mice
- Oryzias
- Toxicity Tests
- Zebrafish
- PubMed
- 34822589 Full text @ Toxins (Basel)
Citation
Sangermano, F., Masi, M., Kumar, A., Peravali, R., Tuzi, A., Cimmino, A., Vallone, D., Giamundo, G., Conte, I., Evidente, A., Calabrò, V. (2021) In Vitro and In Vivo Toxicity Evaluation of Natural Products with Potential Applications as Biopesticides. toxins. 13(11):.
Abstract
The use of natural products in agriculture as pesticides has been strongly advocated. However, it is necessary to assess their toxicity to ensure their safe use. In the present study, mammalian cell lines and fish models of the zebrafish (Danio rerio) and medaka (Oryzias latipes) have been used to investigate the toxic effects of ten natural products which have potential applications as biopesticides. The fungal metabolites cavoxin, epi-epoformin, papyracillic acid, seiridin and sphaeropsidone, together with the plant compounds inuloxins A and C and ungeremine, showed no toxic effects in mammalian cells and zebrafish embryos. Conversely, cyclopaldic and α-costic acids, produced by Seiridium cupressi and Dittrichia viscosa, respectively, caused significant mortality in zebrafish and medaka embryos as a result of yolk coagulation. However, both compounds showed little effect in zebrafish or mammalian cell lines in culture, thus highlighting the importance of the fish embryotoxicity test in the assessment of environmental impact. Given the embryotoxicity of α-costic acid and cyclopaldic acid, their use as biopesticides is not recommended. Further ecotoxicological studies are needed to evaluate the potential applications of the other compounds.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping