PUBLICATION
Effects of parental environmental copper stress on offspring development: DNA methylation modification and responses of differentially methylated region-related genes in transcriptional expression
- Authors
- Tai, Z., Guan, P., Zhang, T., Liu, W., Li, L., Wu, Y., Li, G., Liu, J.X.
- ID
- ZDB-PUB-211123-3
- Date
- 2021
- Source
- Journal of hazardous materials 424(Pt C): 127600 (Journal)
- Registered Authors
- Liu, Jing-xia
- Keywords
- Copper, DNA methylation, Differentially methylated region-related genes, Nervous and digestive system, Transcriptome
- MeSH Terms
-
- Animals
- Copper/metabolism
- Copper/toxicity
- DNA Methylation*
- Epigenesis, Genetic
- Zebrafish*/genetics
- Zebrafish*/metabolism
- Zebrafish Proteins/genetics
- PubMed
- 34801305 Full text @ J. Hazard. Mater.
Citation
Tai, Z., Guan, P., Zhang, T., Liu, W., Li, L., Wu, Y., Li, G., Liu, J.X. (2021) Effects of parental environmental copper stress on offspring development: DNA methylation modification and responses of differentially methylated region-related genes in transcriptional expression. Journal of hazardous materials. 424(Pt C):127600.
Abstract
Parental environmental copper (Cu) exposure is widespread, causing problems for sustainability of fish populations, and epigenetics is suggested to be fundamental during the process, but the mechanism is scarcely reported. Here, we describe the effects of parental environmental Cu exposure on zebrafish developmental abnormality in subsequent generation. This study demonstrated for the first time that: 1. offspring from Cu-stressed paternal adult zebrafish showed developmental defects in the nervous and digestive system and changes in transcriptome; 2. Cu-induced alterations in sperm methylome and transcriptome could induce loci-specific alterations in DNA methylome and corresponding changes in the related gene transcription in offspring; 3. differentially methylated regions in pmpcb, crebl2 and tab2 promoters acted pivotally in their transcription; 4. pmpcb, crebl2 and tab2 are key individual contributors to parental Cu exposure-induced developmental defects in the nervous system, retina and digestive system of the offspring. Those data revealed that Cu-induced alterations in sperm methylome and transcriptome can be passed down to their fertilized offspring, reprogramming the epigenetic and transcriptional regulation of embryogenesis and causing embryonic developmental defects, suggesting that environmental Cu might pose a huge threat to the sustainability of fish populations.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping