PUBLICATION
Phosphinate-benzoindocyanin fluorescent probe for endogenous mitochondrial peroxynitrite detection in living cells and gallbladder access in inflammatory zebrafish animal models
- Authors
- Sonawane, P.M., Lee, W., Kim, Y., Roychaudhury, A., Bhosale, V.K., Kim, D., Park, H.S., Kim, C.H., Churchill, D.G.
- ID
- ZDB-PUB-211116-26
- Date
- 2021
- Source
- Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy 267(Pt 2): 120568 (Journal)
- Registered Authors
- Kim, Cheol-Hee
- Keywords
- Cell imaging, Diphenyl phosphinate, Mitochondria-accessing, ONOO(−), Zebrafish
- MeSH Terms
-
- Animals
- Fluorescent Dyes*
- Gallbladder
- Models, Animal
- Peroxynitrous Acid*
- Reproducibility of Results
- Zebrafish
- PubMed
- 34774434 Full text @ Spectrochim Acta A Mol Biomol Spectrosc.
Citation
Sonawane, P.M., Lee, W., Kim, Y., Roychaudhury, A., Bhosale, V.K., Kim, D., Park, H.S., Kim, C.H., Churchill, D.G. (2021) Phosphinate-benzoindocyanin fluorescent probe for endogenous mitochondrial peroxynitrite detection in living cells and gallbladder access in inflammatory zebrafish animal models. Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 267(Pt 2):120568.
Abstract
Potent oxidants such as peroxynitrite (ONOO-) play important roles in the regulation of different physiopathological processes; their overproduction is thought to potentially cause several diseases in living organisms. Hence, the precise and selective monitoring of ONOO- is imperative for elucidating its interplay and roles in pathological and physiological processes. Herein, we present a novel diphenyl phosphinate-masked benzoindocyanin "turn-on" fluorogenic probe to help detect mitochondrial ONOO- in living cells and zebrafish models. A pale yellow color solution of BICBzDP turns rose-red upon the addition of ONOO-, selectively, contrary to that of other competitive bioactive molecules. BICBzDP displays an ultra-sensitivity detection limit (47.8 nM) with outstanding selectivity and sensitivity towards mitochondrial ONOO- and possesses a notable 68-fold fluorescence enhancement involving a large redshift of 91 nm. Importantly, further biological experimental investigations with BICBzDP indicate specific sensitivity and reliability of the probe to track the ONOO- level, not only in live cells, but also demonstrates dynamic fluctuations in the inflammatory zebrafish animal models. Thus, BICBzDP could be employed as a future potential biological tool for exploiting the role of ONOO- in a variety of different physiological systems.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping